左旋精氨酸甲酯对肝缺血-再灌注损伤的干预及机制.docVIP

参脉注射液对肺缺血-再灌注损伤时Fas/FasL表达的影响 王俊1 林丽娜1＊ 王万铁 2 金立达1 邱晓晓2 戴雍月2 王青2（1.温州医学院附属第一医院麻醉科，温州325000；2.温州医学院病理生理学教研室，温州32500） [摘 要]: 目的 探讨参脉注射液对肺缺血-再灌注损伤（PIRI）时Fas/FasL表达的影响。方法 采用在体兔单肺原位缺血-再灌注模型。实验兔30只，随机分为假手术对照组（sham，10只）、肺缺血-再灌注组（I-R，10只）和肺缺血-再灌注加参脉注射液组（CM，10只）。分别于再灌注3h取左肺组织，观察Fas/Fas配体（Fas/FasL）mRNA定位表达、凋亡指数（AI）、肺组织湿干重比（W/D）、肺损伤组织学定量评价指标（IQA）及光镜、电镜下的组织形态学改变。结果 CM组Fas/FasL mRNA在肺小动脉内（外）膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达，明显低于I-R组（P0.05）；AI、W/D和IQA值显著低于I-R组（P0.01和P0.05）；肺组织形态学异常改变程度减轻。结论 参脉注射液可下调肺组织Fas/FasL mRNA的表达而减轻细胞凋亡，对PIRI发挥积极的防治作用。 [关键词]: 再灌注损伤；肺；Fas/Fas配体；细胞凋亡；参脉注射液 Effect of Shenmai injection on expression of Fas/FasL mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits Wang jun， LIN Li-na*， WANG wan-tie，Jinlida,et al.* Department of Anesthesiology, The First Affiliated Hospital，Wenzhou Medical College， Wenzhou 325000 Zhejiang, China ABSTRACT: OBJECTIVE To investigate the effect of Shenmai injection (SM) on expression of Fas/FasL mRNA during pulmonary ischemia and reperfusion injury (PIRI) in the rabbits. METHODS Single lung ischemia and reperfusion animal model was used in vivo. The rabbits were randomly into three groups ,sham operated group (sham, n=10),ischemia-reperfusion group (I-R, n=10) and I-R+ Shenmai injection group(CM, n=10).Changes of several parameters which included apoptotic index (AI), wet to dry ratio of lung tissue weight(W/D) and index of quantitative assessment of histologic lung injury(IQA) were measured at 180 minutes after reperfusion in lung tissue. Meanwhile the location and expression of Fas / FasL mRNA were observed. lung tissue was prepared for light microscopic and electron microscopic observation at 180 minutes after reperfusion . RESULTS As compared with group I-R, Fas/FasL mRNA slightly 基金项目：浙江省中医药管理局（2005C088）； 作者简介：王俊，男，硕士研究生 ＊通讯作者:林丽娜 ，女，教授，硕士生导师 tel:（0577） e-mail：wzlinlina@ expressed in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia in CM group.