培养条件对阴沟肠杆菌乐果降解效能的影响（.docVIP

培养条件对阴沟肠杆菌乐果降解效能的影响( 官雪芳1，2 刘波1* 林斌2 林抗美1 马丽娜1 （1 福建省农业科学院生物资源研究所，2福建省农科院农业工程技术研究所，福建 福州350003 ,） 摘要： 从福建省福农生化有限公司排污口的土壤中筛选到一株对乐果有较好降解效果的菌株阴沟肠杆菌为了使该菌能更好的应用于乐果残留污染的治理当中特L16（44×23）对该菌降解乐果的作用因素进行结果为：菌降解乐果的最佳发酵条件组合为：碳源：蔗糖离子：CuCl2:(10 Mmol/L)乐果浓度：100mg/L培养时间36h培养温度30℃pH值8氮源：酵母3g/L不同水平对乐果的降解存在显著差异（5%置信度），Orthogonal experiments for optimizing fermentation conditions of Enterobacter closcae to degrade dimethoate GUAN Xuefang1，2,LIU Bo1* LIN Bin2 LIN Kangmei1, MA Lina1, (1Institute of Agro-biological resources，Fujian Academy of Agricultural Sciences),(2 Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences，Fuzhou，350003, China) Abstrat: By using selective cultural media, Enterobacter closcae was isolated to degrade dimethoate from the soil around the pesticide factory. In order to make it better used with residual dimethoate, the L16（44*23）orthogonal test was applicated to study the effective factors of the degradation to imethoate of E. closcae. The results showed that the conformable carbon source for the degradation was sucrose, the ion was Cu2+ with the density of 10 Mmol/L, the density of dimethoate was 100mg/L, the optimum temperature was 30℃, the initial pH was 8.0, the conformable nitrogen source was yeast extract with the density of 3g/L, and the culture time was for 36h. Meanwhile, using the DPS Date Processing System, the single factor effect and sample were statisticed. The most appropriate sample was number5, number16 and number7 with the degrading rates of 58.90%、50.2%、49.47%, also there was a significant difference (5% confidence level) in each sample with the degrading rates of 0-58.90%. The effect of ion has the most significant impact on the degradation of rates, and then followed by pHculture time, density of dimethoate,temperature and carbon source. Key Words: Enterobacter closcae, Dimethoate, Degradation, Optimize orthogonal experiments 有机磷农药一直是我国用量最大的农药种类，每年都在十万吨以上，它在给农业生产带来效益的同时也给人类带来了很大的污染问题，大量的农药施于农田后只有大约