原代神经元转染方法.pdfVIP

· 455 · 生命的化学, 2013, 33(4): 455-460 刘俊燕, 等. 原代神经元转染方法 原代神经元转染方法 1,2 1,2 1,2 * 1,2 刘俊燕 ，赵凤艳 ，屈艺 ，母得志 1 2 ( 四川大学华西第二医院儿科，成都61004 1；妇儿疾病与出生缺陷教育部重点实验室，成都61004 1) , 摘要：体外培养的原代神经元形态和功能与在体神经元相似 在神经系统疾病研究中能提供准确的生 物学信息，是研究神经系统疾病的重要工具。转染技术的发展，为研究原代培养神经元基因和蛋白质 功能提供了技术支持。本文总结了近年来原代神经元转染方法的研究进展，比较了不同转染方法如磷 酸钙沉淀法、脂质体介导转染、电穿孔法、病毒介导转染等的优缺点，为选择最佳的神经元基因转染 技术提供有价值的参考。 关键词：原代神经元；转染方法；磷酸钙沉淀法；脂质体法；电穿孔法；病毒转染 Transfection techniques for primary cultured neurons LIU Junyan1,2, ZHAO Fengyan1,2, QU Yi1,2*, MU Dezhi1,2 1 2 ( Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Obstetric Gynecologic and Pediatric, Diseases and Birth Defects, Ministry of Education, Chengdu 610041, China) Abstract: The primary cultured neurons in vitro have similar morphology and function as those of neurons in vivo. Therefore, it can provide accurate biological informations and is regarded to be an important tool in the studies of nervous system disorders. The development of transfection technology has provided technical supports for functional research of genes and proteins in neurons. Here we will summarize the research progress in transfection methods of primary cultured neurons in recent years, and will compare the advantage and disadvantage among different transfection methods such as calcium phosphate method, lipo