大蒜及双氧水降解内毒素的作用原理研究.pdfVIP

中华口腔医学杂志2000 年 9 月第 35 卷第 5 期 　Chin J Stomatol , September 2000 , Vol 35 , No. 5 3·33 · · · 论著 大蒜及双氧水降解内毒素的作用机理研究 李德懿 　焦炳华 　朱玉平 　汪俊 ( ) 　　【摘要】　目的　阐明大蒜及双氧水降解内毒素 lipopolysaccharide , LPS 的作用机理 。方法 　采 用热酚水法 、苯酚氯仿石油醚法 、鲎试验 、Lowry 灰迹光谱法及气相色谱等方法 ,分析大蒜 、双氧水降 解LPS 的量效与时效关系及结构变化 。结果 　30 %双氧水降解作用最大 ,其余依次为大蒜原汁 、大蒜 1 ∶1 稀释液及 3 %双氧水 ,作用具浓度依赖性 ,大蒜组还有时间依赖性 。实验证实 30 %双氧水能裂解 LPS 类脂 A 还原端 C1 位上的磷酸基团 ,而大蒜作用机理较复杂 ,除水解作用外 ,还能螯合 LPS 分子 , 影响LPS 单体发挥作用 。结论 　各种药物降解LPS 的部位和机理不同。 【关键词】　内毒素类 ; 　生物降解 ; 　大蒜 Effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide 　LI Deyi , JIAO Binghua , ZHU Yuping , et al . Department of Oral Medicine , School of Stomatology , Shanghai Second Medical University , Shanghai 200011 , China 【Abstract 】　Objective 　To elucidate the effect and mechanism of garlic juice and hydrogen peroxide on the degradation of lipopolysaccharide (LPS) . Methods 　Hot phenolwater method , phenolchloroformpetroleum ether procedure , limulus lysate test , lowry′s ash spetrographical examination and gasliquid chromatography etc. were used in this study. Results 　The sequence of degradation effect was 30 % hydrogen peroxide ( H) , the most ( ) powerful ,followed by garlic juice G , 1 ∶1 diluted G and 3 % H , their effects were dose dependent and G group was time dependent. The mechanism of H on LPS degradation was fractionization of phosphoryl in position 1 from lipid A , while that of G was complex , it could bound LPS molecule and influenced its effect besides LPS hydrolysis. Conclusion 　The study may imply that