大鼠口服木通皂苷D粪便中代谢物的HPLC-MS^n分析.pdfVIP

LIKai．YANGXiao—Lin ，ZHANGChun—Feng，YANGZhong—Lin KeyLaborato~ofModernChineseMedicines，Minist~ofEducation，ChinaPharmaceuticalUniversity,Nanjing210009 DepartmentofBiologicalandPharmaceuticalEngineering，WuhanPolytechnicUniversity,Wuhan430023，China A【BSTRACT】 AII：ToinvestigatethemetabolitesofasperosaponinVIinrats’feces．METHODS：FecesWerecol— lectedafteroraladministrationof400mgkg～asperosaponinVItorats．ThesamplesweredetectedbyLC—M S method． RESULTS：Majormetaboliteswerefound inratfecesand theirchemicalstructureswereidentifiedprimarily． C0NCLUSION：Themetabolitesfoundinfeceswere3-O——L—arabinopyranosylhederagenin一28--D—glucopyranoside， hederagenin．28 ．D—glucopyranoside，3-O—a-L—arabinopyranosylhederagenin，hederagenin，heder—agenin一28 ，J—glu— copyranoside一(1—}6)一∥一D—glucopyran0side． 【KEYWOlIDS】AsperosaponinVI；Feces；Metabolites；HPLC—MS 【CLCNumberlR969．1 [Documentcode]A [ArticleIDI1672—-3651(2009)06—．0440-0·4 doi：10．3724／SR J．1009．2009．00440 1 IntrOductiOn chromatography(HPLC)一electrosprayionization(ESI)一MS“ methodto identifythemetabolitesofasperosaponinVIin Asperosaponin VI r3一O—d—L—arabinopyranosylhede— ratsfeces． ragenin一28 一D—g1ucopyranoside一(1__+6) 一D—glucopyrano— side)，also named akebia saponin D，isacharacteristic 2 Experimental triterpenoidsaponin isolatedfrom therhizomeofDipsacus 2．1 ChemicaIsandreagents PrWall(Dipsacaceae)．Ithaslongbeenusedasatonic Asperosaponin V1wasisolated from Dipsacusas． and anti～inflammatory agentin TraditionalChineseM edi— peroidesattheauthor’s1aboratory．anditsstructurewas