枯雷尔氏菌的色谱行为表征及防效指数色谱效价的构建.docVIP

植物疫苗工程菌青枯雷尔氏菌色谱表征及防效指数色谱效价构建 刘波1，郑雪芳1，余谦1,2 （1福建省农业科学院农业生物资源研究所，福建 福州 3500031；2福州大学生命科学学院，福建 福州 350008；） 关键词：高效液相色谱法；青枯雷尔氏菌；生防菌 The Ralstonia solanacearum chromatographic behavior characterization and anti-efficiency analysis Liu Bo1*, Zheng Xuefang1 ,Yu Qian1,2 （1Biotechnology Institution, Fujian Academy of Agricultural Sciences, Fuzhou 350003 2College of Life Science Fuzhou university, Fuzhou 350108, China;） Abstract：Ralstonia solanacearum, a widely distributed pathogen, was characterized by high-performance liquid chromatography (HPLC). The pure culture of Ralstonia solanacearum was successfully separated into one 、two or three characteristic fractions. The rates were 54.1%, 16.2% and 29.7%. The results showed that strong virulent strains formed only one characteristic peak at the retention time of 17 minute. In the bio-control test, ten Ralstonia solanacearum strains’ control effect more than 30%.The best strain was T838, which reached 70.8%. Twenty-one strains were analyzed by HPLC and applied to the experiment of Biological control effect. The results showed that there are eight better control effect strains formed only one characteristic peak. As the result, we may conclusion that most of better control effect strains formed only one characteristic peak. The results are very important to elucidate the mechanism of Ralstonia solanacearum’S pathogentic mutation and screening excellent bio-control bacteria． Key words：Chromatography-potency; Ralstonia solanacearum; Plant vaccine for bio-control bacterial wilt disease 青枯雷尔氏菌(Ralstonia solanacearum)，属于革兰氏阴性细菌，其引起的植物细菌性青枯病害(Bacterial wilt)，简称青枯病，是一种世界范围的细菌性土传病害。该菌寄主广泛，能够侵染多达450种以上的植物种类，严重危害很多重要经济作物，如番茄、烟草、辣椒等[1]。该病原菌从寄主植物的根部，侵入木质部导管，通过维管束系统迅速扩张到植物的地上部分，病菌的大量定殖引起导管功能丧失，进而引发植物萎蔫，导致植物死亡[2]。 由于青枯雷尔氏菌在土壤中的存活时间久，传播快，目前还尚未有很好的防治措施。传统的防治方法是化学防治，不但污染环境严重，而且防效。土传病害青枯病的生物防治引起科学家的重视青枯雷尔氏菌。青枯雷尔氏菌存在致病力分化，在同一个环境下存在强致病力菌株和无致病力菌株，前者能引起寄主植物发生青枯病，后者保持了侵染特性，但不引起寄主植物发病[3]。前人的研究报道了将青枯雷尔氏菌无致病力菌株作为防治青枯病，取得良好的防效[4]。刘波提出了利用青枯雷尔氏菌无致病力菌株作为植物疫