提高外源基因在转基因植物中表达效率的途径.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2005,13 (6): 808~814 ·综述· 提高外源基因在转基因植物中表达效率的途径* 1 1 2 3 涛 张上隆 ** 刘敬梅 陈大明 (1. 浙江大学园艺系，杭州310029 ；2. 清华大学生物系，北京 100084 ； 摘要：利用转基因技术获得具有目的性状的转基因植株，是进行作物品种改良的有效方式。但基因沉默等现象降低了外源 基因在转基因植物中的表达效率。从外源基因的DNA 修饰水平、翻译后蛋白的定位、以及转基因方式 方面综述了国内外植 物基因工程中的相关研究; 分析了外源基因表达效率低的原因；提出了克服基因沉默并实现外源基因高效表达的途径。 关键词: 转基因植物，外源基因，表达效率 Methods of Improving Heterogeneous Gene Expression Level in Transgenic Plants 1 1 2 3 YIN Tao ZHANG Shang-Long ** LIU Jing-Mei CHEN Da-Ming With the development of plant genetic engineering, many transformation methods can be used to transfer heterogeneous genes with economic merit into plants for developing genetic crops. However, a lot of research results showed that transgene expression remains largely unpredictable and there is a great variation of expression level in plants. The researches of plant genetic engineering were reviewed in the paper . We analyzed the course of low efficiency of heterogeneous gene expression in DNA modification level, the location of pre-protein and the methods of genetic transformation, then gave some advices in improving heterogeneous gene expression with high efficiency in transgenic plants. transgenic plants; heterogeneous gene; expression level 自从 1983 年首例转基因植株问世以来，一大批 关研究结果，提出了如何提高外源基因在转基因植 具有重要 艺性状的基因通过基因枪或 杆菌介导 株中高效稳定表达的几种策略，为以后的研究提供 法转入植物得到表