高压乳匀法制备中药固体脂质纳米粒[J].docVIP

高压乳匀法制备中药固体脂质纳米粒 作者：厉英超1；董蕾2；贾皑1；苌新明1；薛挥1　　(西安交通大学医学院1第一附属医院消化内科，陕西 西安710061；2第二附属医院消化内科，陕西 西安 710004) 摘要：目的??采用高压乳匀法将中药有效成分包载于固体脂质纳米粒(SLN)，并研究制备的纳米粒的主要性质。方法??选择水飞蓟宾(SIL)和汉防己甲素(TET)为模型药物，采用高压乳匀法将其分别包载于SLN。在电镜下观察其形态，以粒度分析仪和Zeta 电位分析仪测定其粒径和Zeta电位，用葡聚糖凝胶柱层析法和HPLC测定其包封率和载药量，还观察了SLN的稳定性。结果??高压乳匀法制备的SIL-SLN呈球状，形态规则，平均粒径为(157±8) nm，Zeta电位为(-35.36±2.68)mV ，包封率为95.64%，载药量为4.63%；TET-SLN呈片状存在，不规则，粒径较小，平均粒径为(47±3) nm，Zeta电位为(-32.99±2.54)mV，包封率为97.82%，载药量为4.76%。SIL-SLN和TET-SLN有较高稳定性。结论??高压乳匀法适于制备包载中药的SLN。 关键词：水飞蓟宾；汉防己甲素；固体脂质纳米粒；高压乳匀法；中药现代化 中图分类号：R944??文献标识码：A??文章编号：1673-4254(2006)05-0541-04 PreParation of solid lipid nanoParticles loaded with traditional Chinese medicine by high-pressure homogenizationLI Ying-chao1；DONG Lei2；JIA Ai1；CHANG Xin-ming1；XUE Hui11DePartment of Gastroenterology,First Affiliated Hospital,Medical College of Xi’an Jiaotong University,Xi’an 710061,China;2DePartment of Gastroenterology,Second Affiliated Hospital,Medical College of Xi’an Jiaotong University,Xi’an 710004,ChinaAbstract:Objective??To investigate the preParation of solid lipid nanoParticles (SLN) loaded with traditional Chinese medicines by high-pressure homogenization,and study the physicochemical characteristics of the Particles produced by this method.Methods??The model traditional Chinese medicines,silibinin (SIL) and tetrandrine (TET), were incorporated into SLN seParately by high-pressure homogenization. Transmission electron microscope was employed to study the shape of the Particles. Particle characterization system and zeta potential analyzer were used to study the diameter and zeta potential of SLN in the suspension. The entrapment efficiency and drug loading were determined with the sephadex gel chromatography and high-performance liquid chromatography. The stability of SLN was also studied. Results??The SIL-SLNs prePared by high-pressure homogenization were spherical and regular. The mean diameter and zeta potential of SIL-SLN in distilled water were 157±8 nm and -35.36±2.68