虎刺醛对人肝癌细胞HepG2生长抑制、细胞迁移抑制及其机制的研究.docVIP

虎刺醛对人肝癌细胞HepG2生长抑制、细胞迁移抑制及其机制的研究 丁 兰( 柳志军 令利军 贾凌云 第五佳丽 彭 舒 刘国安 (西北师范大学生命科学学院, 兰州 730070) 摘要台盼蓝检测了虎刺醛对人肝癌细胞HepG2的生长、细胞迁移、微丝和角蛋白的影响。结果表明, 15~μmol/L的虎刺醛对HepG2细胞有生长抑制作用, 浓度时间依赖性; 15 μmol/L)的虎刺醛能够抑制HepG2细胞迁移, 30 μmol/L几乎完全抑制迁移; 2527.5, 30, 35 μmol/L虎刺醛作用HepG2细胞24 h, 其细胞内的应力纤维、片状伪足、丝状伪足逐渐减少或消失μmol/L细胞松弛素B的处理结果相似; 而25 μmol/L和30 μmol/L虎刺醛HepG2细胞; 15~60 μmol/L的虎刺醛能诱导HepG2细胞发生凋亡。虎刺醛HepG2细胞内应力纤维(或微丝)的破坏作用极可能是导致细胞生长抑制、迁移抑制的直接原因, 也是使细胞贴壁性降低或丧失从而导致凋亡发生的直接原因。 关键词虎刺醛; HepG2; 细胞迁移; ; 角蛋白; 细胞凋亡of Damnacanthal on Anti-proliferation, Cell Migration Inhibition and Its Mechanism of Human Hepatoma Cell HepG2 Ding Lan(, Liu Zhijun, Ling Lijun, Jia Lingyun, Diwu Jiali, Peng Shu, Liu Guoan (College of Life Science, Northwest Normal University, Lanzhou 730070, China) Abstract The trypan blue staining, wound healing assay, specific fluorescence staining are used to investigate the effect of proliferation, cell migration, microfilaments cytoskeleton, keratin filaments network and apoptosis induced by damnacanthal on human hepatoma cells HepG2 respectively. The results demonstrated that damnacantha1 significantly inhibited cell proliferation of human hepatoma cell HepG2 at concentration of 15~60 μmol/L in a time and dose dependent manners; 15 μmol/L damnacanthal inhibits cell migration and 30μmol/L almost inhibits completely; after HepG2 cells were exposed to damnacanthal (25, 27.5, 30, 35 μmol/L) for 24 h, stress fibers, lamellipodia and filopodia were absent or decreased gradually with increased concentration, this similar to the results caused by 8μmol/L cytochalasin B in the same condition; however, 25 μmol/L and 30 μmol/L damnacanthal were only slightly influence on keratin filaments of HepG2; 15~60 μmol/L damnacanthal induced apoptosis of HepG2 in differently degrees. The destructive effects of damnacanthal on HepG2 cells stress fiber (microfilaments) may be the direct reason of anti-proliferation and cell migration inhibition, may be the direct reason of cell adherence decreased