卵巢癌细胞与巨噬细胞共培养对B7-H1表达影响及机制.pdfVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(12): 1760– 1764 DOI: 10.11844/cj cb.2013.12.0222 卵巢癌细胞与巨噬细胞共培养对B7-H1 表达的影响及机制 熊海玉 马婷婷 王 秦 董晋豫 梁勤东 李紫微 张维理 涂植光* (重庆医科大学检验医学院, 临床检验诊断学教育部重点实验室, 重庆400016) 摘要 为了探讨卵巢癌细胞与巨噬细胞共培养后对B7-H 1表达的影响及其可能机制, 利用佛 波酯(PMA)诱导THP-1或外周血单核细胞分化为巨噬细胞后, 与人卵巢癌细胞株SKOV3体外非接 触共培养24 h, qRT-PCR 、Western blot 以及流式细胞术分别检测SKOV3与巨噬细胞B7-H 1的表达; κ 进一步利用NF- B 、JAK2/STAT3、p38 MAPK信号通路的抑制剂作用于共培养体系, 检测B7-H 1 表达的变化, 以探讨其机制。结果显示, 共培养24 h后, SKOV3及巨噬细胞B7-H 1 mRNA和蛋白 P κ 的表达较非共培养组均显著升高( 0.05), 而阻断NF- B 、JAK2/STAT3、p38 MAPK信号通路后, B7-H 1的上调均明显被抑制(P0.05) 。SKOV3与巨噬细胞共培养后B7-H 1的表达升高(P0.05), 其 κ 机制可能涉及到NF- B 、JAK2/STAT3、p38 MAPK信号通路的激活。 关键词 卵巢癌; B7-H 1; 肿瘤相关巨噬细胞 The Effects of Ovarian Cancer Cells Co-cultured with Macrophages on the Expression of B7-H1 and Its Mechanisms Xiong Haiyu, Ma Tingting, Wang Qin, Dong Jinyu, Liang Qindong, Li Ziwei, Zhang Weili, Tu Zhiguang* (College of Laboratory Medicine, Key Laboratory of Laboratory Medical Diagnostics of Education Ministry, Chongqing Medical University, Chongqing 400016, China) Abstract To investigate the effects of ovarian cancer cells co-cultured with macrophages on the expre- ssion of B7-H1 and its possible mechanisms, phorbol 12-myristate 13-acetate (PMA) treated THP-1 cells or hu- man monocytes were co-cultured with human ovarian cancer cell line SKOV3 for 24 h in vitro without direct contact, the expression of B7-H1 in SKOV3 cells and macrophages was detected by qRT-PCR, Western blot and flow cytometry, respectively; In addition, the expression of B7-H1 was also determined after treating the cocul- ture system