双水相超声波法辅助提取甜玉米芯多酚及抑菌性研究.pdfVIP

※工艺技术 食品科学 2013, Vol.34, No.24 61 双水相超声波法辅助提取甜玉米芯多酚及 抑菌性研究 马永强，荆丽荣，刘晓飞，张 娜，刘晓凝 (哈尔滨商业大学 黑龙江省普通高等学校食品科学与工程重点实验室，黑龙江 哈尔滨 150076) 摘  要：通过单因素和正交试验，研究利用双水相超声波辅助提取甜玉米芯多酚类化合物的工艺。考察丙酮体积 分数、硫酸铵用量、提取时间、料液比4个单因素对多酚提取率的影响。结果表明：通过正交试验得到多酚提取 的最佳工艺为丙酮体积分数60%、硫酸铵用量0.175g/mL 、提取时间25min 、料液比1:20(g/mL) ，此条件下所得提 取液的多酚提取率为4 .4 1% 。甜玉米芯多酚提取物有抑菌活性，对大肠杆菌、枯草芽孢杆菌、白葡萄球菌抑制作 用明显。 关键词：甜玉米芯；多酚；超声波提取；抑菌活性 Ultrasonic-Assisted Aqueous Two-Phase Extraction and Antimicrobial Activity of Sweet Corncob Polyphenols MA Yong-qiang，JING Li-rong，LIU Xiao-fei ，ZHANG Na，LIU Xiao-ning (Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China) Abstract ：One-factor-at-a-time and orthogonal array designs were applied to establish for optimal conditions for ultrasonic- assisted aqueous two-phase extraction of polyphenols from sweet corncob. The effects of four process parameters including acetone concentration, ammonium sulfate dosage, extraction time and solid-to-liquid ratio on the yield of polyphenols were investigated. The optimal extraction conditions were found as follows: an aqueous two-phase system formed by adding 0.175 g/mL of ammonium sulfate to 60% aqueous acetone, solid-to-solvent ratio of 1:20 (g/mL), and 25 min extraction. The yield of polyphenols in extracts under these conditions was 4.4 1%. The polyphenols extracted from sweet corncob had significant antibacterial activity against Escherichia coli, Bacillus subtilis and Staphylococcus white. Key words ：sweet corncob ；polyphenols；ultrasonic-assisted extraction ；antibacterial activity 中图分类号：TS209 文献标志码：A 文章编号：1002-6630(2013)24-0061-04 doi:10.7506/spkx 1002-6630-20 13240 12 甜玉米