灰树花多糖对小鼠四氯化碳肝损伤保护作用.docVIP

灰树花多糖对小鼠四氯化碳肝损伤的保护作用研究 刘丰韬1 许林1张媛媛2 刘甜甜2冯玉新3辛华3李霞3 【摘要】 目的：初步探讨灰树花多糖（Grifola Frondosa Polysaccharide，GFP）对小鼠四氯化碳（CCl4）肝损伤的保护作用。方法：将70只昆明种小鼠随机分为正常对照组、CCl4损伤组和损伤保护组（灰树花多糖灌胃浓度分别为7.5μg/kg、15μg/kg、30μg/kg、60μg/kg和120μg/kg五个梯度）。采用CCl4腹腔注射法制备小鼠急性肝损伤动物模型，灰树花多糖灌胃给药建立保护模型。通过分析小鼠体重、脏器体重比改变，肝组织石蜡切片HE染色、冰冻切片碱性磷酸酶(AKP)染色的组织病理学变化以及血清谷丙转氨酶（ALT）、谷草转氨酶（AST）活性变化，探讨灰树花多糖对小鼠CCl4急性肝损伤的保护作用。结果:灰树花多糖可明显改善CCl4所致小鼠肝组织充血、水肿及肝细胞变性现象，可维持组织AKP含量，降低血清中ALT、AST含量，对肝脏起保护作用。结论：灰树花多糖对小鼠CCl4肝损伤具有明显保护作用，综合评价最佳保护浓度为30μg/kg。 【关键词】 灰树花多糖；CCl4；肝损伤；保护作用；碱性磷酸酶；小鼠 Protective Effect of Grifola Frondosa Polysaccharide on the Hepatic Injury of Mice Induced by CCl4 LIU Feng-tao1 XU Lin1 ZHANG Yuan-yuan2 LIU Tian-tian2 FENG Yu-xin3 XIN Hua3 LI Xia3 Clinical Medicine (6 years), 2. Medical Image (6 years), 3. Institute of Cell Biology, School of Medicine, Shandong University, Jinan, 250012, China) 【Abstract】 Objective: To investigate the protective effect of Grifola Frondosa Polysaccharide (GFP) on the acute hepatic injury of mice induced by CCl4. Methods: The 70 mice were divided into seven groups randomly, including a control group, an injury group and five GFP treated groups with the GFP concentration of 7.5μg/kg、15μg/kg、30μg/kg、60μg/kg and 120μg/kg. The mouse acute liver injury model was induced by carbon tetrachloride (CCl4). The protective effect of GFP on the mice hepatic injury is evaluated by the change of the weight, organ/weight ratio, the pathological morphology, the alkaline phosphate (AKP) histochemical stain of the frozen slice, as well as the enzyme function of serum. Result: The increase of ALT, AST in serum, and the decrease of AKP in tissue, which were induced by CCl4, could be antagonized by GFP. The hepato-cellular degeneration and necrosis induced by CCl4 could also be diminished. Conclusion: GFP can protect the hepatic injury of mice induced by CCl4 predominantly, and the optimal dosage is 30μg/kg after synthesis. 【Key Words】 Grifola Frondosa Polysaccharide; CCl4; hepatic injury; Alkali