青海湖嗜盐菌分离与优势菌株QHL5特性.pdfVIP

2012-10-25 DOI: 10.3724/SP.J.1145.2012.00797 http:// 应用与环境生物学报 Chin J Appl Environ Biol 2012, 18 5 : 797~803 * 青海湖嗜盐菌的分离与优势菌株QHL5的特性 1** 2 1 1 1 1 3 朱 德 锐 刘 建 刘 静 静 沈 国 平 龙 启 福 高 翔 刘 德 立 1 (青海大学医学院 西宁 810016) 2 ( 华中师范大学生命科学院 武汉 430079) 3 ( 湖北省遗传调控与整合生物学重点实验室 武汉 430079) 摘 要采用高盐选择性培养基从20份青海湖水样中筛选分离获得35株青海湖嗜盐微生物. 耐盐梯度实验分析表明: 水体中以中度嗜盐菌为主, 约占65.7%; 弱嗜盐菌次之,约占25.7%; 非嗜盐菌与耐盐菌相对较少,约占8.5%. 其中优势菌 QHL5的生长盐度耐受范围为0.04~3.0 mol/L, 最适生长范围为0.3~0.9 mol/L, 隶属于中度嗜盐菌. 菌体形态和生长特性 分析表明:QHL5菌落大小为4~6 mm, 圆形或椭圆形, 边缘整齐厚实, 乳白色, 中间微隆起, 不透明, 革兰氏染色阴性显微形态呈杆状, 最适生长pH为8.5~9.5, 最适生长温度为35 ℃. 生理生化、 16S rRNA的全序列Blast和系统发育分析表 明:QHL5菌株分类定位于γ变形菌纲海洋螺菌目盐单胞菌属樊氏盐单胞菌(Halomonas ventosae). 通过80%乙醇法抽 提胞内相溶物质, 采用TLC法检测出该菌积聚四氢嘧啶. 图6 表1 参28 关键词青海湖; 中度嗜盐菌;分子鉴定;樊氏盐单胞菌; TLC法; 四氢嘧啶 CLC Q939.106 Isolation of Halophiles from Qinghai Lake and Growth Characteristics of * Dominant Strain QHL5 1** 2 1 1 1 1 3 ZHU Derui , LIU Jian , LIU Jingjing , SHEN Guoping , LONG Qifu , GAO Xiang LIU Deli 1 Medical College, Qinghai University, Xining 810016, China 2 College of Life Sciences, Central China Normal University, Wuhan 430079, China 3 Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Wuhan 430079, China AbstractThirty-five strains of halophiles were isolated from 20 water samples of Qinghai Lake, China by a high saline medium. In all strains tested, the proportion of the moderate halophile was 66.7%, as a dominate group, and the percentage of the slight halophile was 25.7. The nonhalophile plus halotolerant was however only 8.5%. The dominant strain QHL5 was a moderate halophile, which could grow in a salt concentration from 0.04 to 3.0 mol/L, and its optimal concentration was 0.3~0.9 mol/L. According to colonial morphology and growth characteristics, QHL5 was round or oval, 4.0~6.0 mm, white, gram negative and opaque as a single cell. The optimum pH and cultural temperature were 7.5~8.0 and 35 ℃, respectivelyBased on the physiological and biochemical characteristics of bacteria, and phyl