氮元素对发菜生长及多糖含量的影响.pdfVIP

92 2010, Vol. 31, No. 19 食品科学 ※基础研究 氮元素对发菜生长及多糖含量的影响 1,2 2 1, 汤 俊 ，万 能 ，胡征宇 * ( 1.中国科学院水生生物研究所，湖北 武汉 4 30072 ；2 .常熟理工学院生物与食品工程系，江苏 常熟 2 15500) 摘 要：为优化培养条件，研究含氮(BG 11)和无氮(BG 110)培养基对发菜(Nostoc f lagellif orme) 的色素、藻胆蛋白及 胞内多糖和胞外多糖(EP S)含量的影响。结果显示：含氮组发菜的叶绿素a(Ch l a) 、类胡萝卜素(Caroten oid s) 、藻 蓝蛋白(P C) 、别藻蓝蛋白(A P C) 、藻红蛋白(PE ) 的含量均显著高于无氮组；胞内及胞外多糖含量无氮组高于含氮 组，两者胞内多糖含量最大分别为1.03mg/mL 和0.78mg/mL ，EPS 含量最大分别为243.8 μg/mL 和74.9 μg/mL 。表明 氮元素的缺乏对发菜的色素合成有抑制作用，但在一定程度上能够促进胞内及胞外多糖的合成和分泌。 关键词：发菜；氮；多糖；藻胆蛋白 Effect of Nitrogen on the Growth and Polysaccharide Content of Nostocf lagellif orme 1,2 2 1, TANG Jun ，WAN N en g ，HU Zheng-yu * (1.Institute of Hydrobiology , Chinese Academy of Sciences, Wuhan 430072, China； 2. Department of Biology and Food Engineering, Changshu Institute of Technology, Changshu 2 15500, China) Abstract：Cultivation of Nostocf lagellif orme in nitrogen-containing (BG 11) and non-nitrogen-containing (BG 110) media was conducted for figuring out the effects of nitrogen on the growth and the contents of phycobiliprotein (PC), allophycocyanin (APC), phycoerythrin (PE) and endo- and exopolysaccharides of Nostocf lagellif orme were investigated. The results showed that after 20 d cultivation, compared to BG 110 medium, N.f lagellif orme cultured in BG11 medium had much higher contents of Chl a, carotenoides, PE, APC and PC, but much lower contents of endo- and exo poly saccharides. The maximum content of endopolysaccharides was 1.03 mg/mL in BG 110 and 0.78 mg/mL in BG11, while that of exopolysaccharides was 243.8 μg/mL in BG 110