宏基因组学的研究进展.pdfVIP

安徽农业科学袁允燥怎则灶葬造 燥枣 粤灶澡怎蚤 粤早则蚤援 杂糟蚤援 圆园园8袁36渊2冤院415-416袁479 责任编辑 胡剑胜 责任校对 淼 宏基因组学的研究进展 冯美琴1袁2 金陵科技学院动物科学与技术学院 江苏南 南 农业大学食品科技学院 江苏南 渊 1. 袁 210038 曰2. 袁 210095冤 摘要 宏基因组学尝试通过免培方法获得微生物的纯培养 主要技术包括 的提取 文库的构建和目标基因克隆的筛选 可用于 袁 DNA 尧 袁 发现新基因 开发新的生物活性物质 研究群落中微生物多样性等方面 笔者对宏基因组学技术的方法和应用作了简要综述 尧 尧 遥 遥 关键词 宏基因组学 未培养微生物 文库构建 文库筛选 曰 曰 曰 中图分类号 Q78 文献标识码 A 文章编号 0517-6611 2008冤02-00415-02 Research Progress of Metagenomics FENG Mei蛳qin (College of Animal Science and Technology, Jinling Institue of Technology, Nanji ng, Jiangsu 210038) Abstract About 99 % microorganisms in natural environment cannot be obtained their pure culture by traditional isolation and culture methods, which limits the exploitation and application of microbial resources. Metagenomics tries to overcome this bottleneck by developing and using culture 蛳independent approaches, and it offers enormous scope and potential for microbiology. It comprises isolation of DNA, library construction and screening of target gene clone, and can be used in the discovery of new gene and biocatalysts and in the study of microbial biodiversity in a community. The methodology and application of metagenomics have been briefly summarized. Key words Metagenomics; Uncultured microorganisms; Library construction; Library screening 微生物是地球上已知种类最多 数量最大 分布最广的 样品总 的提取 宏基因组文库构建的关键之一 尧 尧 2.1.1 DNA 遥 生物类群 仅原核微生物的总量大约就达 30 30 个[1] 是获得高质量的目的样品的总