乙醇预处理对肝脏缺血再灌流损伤的保护作用X.pdfVIP

消化外科　2004 年第 3 卷第 3 期 207 - 210 　　 ( ) 2·07 · Journal of Digestive Surgery , 2004 ,3 3 :207 - 210 ( ) 文章编号 : 1671 - 4555 2004 03 - 0207 - 04 ·实验研究· 乙醇预处理对肝脏缺血再灌流损伤的保护作用 冯晓彬 　郑树国 　马正伟 　李昆 　董家鸿 　　【摘要】　目的 　探讨胃饲乙醇预处理对大鼠肝脏缺血再灌注损伤的影响。方法 　雄性 Wistar 大 鼠随机分组,动物乙醇预处理后制作缺血再灌注模型 ,于术后 0 到 72 h 内不同时相活杀大鼠,留取血 及肝脏标本保存待检。结果 　实验组和实验对照组较正常组ALT 值明显升高 ,72 h 后基本恢复 ,实验 组恢复较快 ,但实验组和对照间统计学差异不明显 ;病理观察见实验组较对照组表现为较轻的病理损 害 ;免疫组化结果 :正常肝脏仅见弱阳性 ,散在分布于胞浆 ;术后随再灌注时相的延长 ,HSP70 表达逐 渐增强 ,实验组表达明显强于对照组和正常组( P 0. 05) ;Western Blotting 显示 HSP70 阳性条带于再 灌注 3 h 开始增强 ,6 h 明显增强 ,持续至 72 h 达到最强 ,实验组和对照组相差显著。结论 　胃饲乙醇 预处理能够明显增强缺血再灌注大鼠肝脏 HSP70 的表达 ,从而提示与较好肝脏保护相关。 　　【关键词】　胃饲 　　大鼠 　　肝脏 　　乙醇 　　预处理 　　缺血再灌注 　　HSP70 　　【中图法分类号】　R657. 3 　　　　【文献标识码】　A Protective effect of ethanol conditioning against ischemiareperfusion injuries in rat liver 　Feng Xiaobin , Zheng Shuguo , Ma Zhengwei , Li Kun , Dong Jiahong . Institute of Hepatobiliary Surgery , Southwest Hepatobiliry Surgery Hospital , Third Military Medical University , Chongqing 400038 　　【Abstract 】　Objective 　To investigate the expression of HSP70 in rat liver preconditioned by ethanol and its influences on the liver ischemiareperfusion injury. Methods 　Adult male Wistar rats were divided randomly into two groups. Alcohol were given though gastric gavage 24 hours before 30minute ischemia in rat liver in the experimental group. Rats were killed at different time points during the period of reperfusion. Blood and liver tissue samples were taken for examinaton. Results 　( 1) There were obvious differences in pathological observation be tween experimental group and control group. Despite of a certain degree of inflammatory cell infiltration , there was no obvious necrosis in exper