快速检疫中培养松材线虫的冷藏真菌菌种筛选.pdfVIP

第 44卷 第 12期 林 业 科 学 Vo1．44．No．12 2008年 12月 SCIENTIA SILVAE SINICAE Dec．，2008 快速检疫中培养松材线虫的冷藏真菌菌种筛选 李海燕 黄北英 熊惠龙 宋玉双 (国家林业局森林病虫害防治总站 沈 阳 110034) 关键词 ： 松材线虫；分散型 Lm幼虫；冷藏真菌培养 ；快速检疫检验 中图分类号：$763 文献标识码 ：A 文章编号：1001—7488(2008)12—0157—06 Selection ofthecold—stored fungusspeciesforculturingBursaphelenchus xylophilusin rapid quarantine LiHaiyan HuangBeiying XiongHuilong SongYushuang (GeneralStationofForestPestManagement，StateForestryAdministratoin Shenyang110034) Abstract： A longprocedureisneededtoquarantinepinewoodnematodesbecauseonlythedispersalthird—stagelarvaecouldbe isolatedanditcannotbeidentifieddirectlybefore beingculturedtoadultnematodes．Tosho~entheprocedure，theeffectsof differenttemperaturesandculturedurationsonthedevelopmentofB ．xylophiluslarvaewere studieswiththreefungusspeciesas theculture media．Theresultshowedthattemperaturehaddifferentialinfluenceondevelopmentofthelarvaeinthethreefungus media，andtheinfluencewasnotsignificant．ThereWas significantlydifferenteffectofthecold-storedfungimediaonthelarvae development．Fusarium sp．wasthebest，Pestalotia sp．wasthesecond，Botrytiscinereawastheworstinpromotinglarvae development．Cold—storedB．cinerea wasnotsuitablefortherapidquarantineofthenematode．When theLm larvaeofB． xylophiluswere abundantthequarantinecouldbeaccomplishedin48hbyculturingtheLⅢ larvaewithcold-storedFusarium sp． ． orPestalotia sp．at30 oC Keywords： pinewoodnematode；thedispersalthird·stagelarvae；culturewiththecoldstoredagefungi；rapidinspection 松材线虫病是一种通过媒介昆虫携带松材线虫(Bursaphelenchusxylophilus)侵染松树而导致松树迅速死 亡的一种毁灭性传染病害。人为调运未经处理或处理不彻底的感病原木、木材、薪材及包装箱等制