采用液相色谱-质谱法测定城市污水样品中的一些内分泌干扰物--外文翻译.docVIP

附 录 Determination of some endocrine disrupter chemicals in urban wastewater samples using liquid chromatography–mass spectrometry Abstract：An analytical method for the simultaneous determination of trace amounts of fourteen endocrine disrupter chemicals (EDCs) in urban wastewater samples has been developed. The studied compounds were: bisphenol A and its chlorinated derivatives (monochloro, dichloro, trichloro and tretrachlorobisphenol A), three alkylphenols (4-n-nonyl, 4-n-octyl and 4-(tert-octyl) phenol) and six well known phthalate esters (dimethyl, diethyl, di-n-butyl, butylbenzyl, bis(2-ethylhexyl) and di-n-octyl phthalate). The method involves extraction from the samples and preconcentration of the analytes using a solid-phase extraction (SPE) procedure and subsequent liquid chromatographic separation with mass spectrometric detection (LC-MS). Bisphenol F was used as a surrogate. Quantification limits found ranged between 12ngL-1 for diethylphthalate and 69ngL-1for 4-(tert-octyl) phenol. The method was satisfactorily used for determination of these chemicals in urban wastewater samples of Granada City (Spain) and validated using a recovery assay with spiked samples. Keywords: Alkylphenols; Bisphenol A; Bisphenol A chlorinated derivatives; Phthalate esters; Liquid chromatography–mass spectrometry (LC–MS); Wastewater analysis 1. Introduction In recent years, a specific category of human made chemicals, called commonly endocrine disrupter chemicals (EDCs), has attracted a great deal of public and scientific attention because of their suspected carcinogenic and estrogenic properties. In 1996, the European Union (EU) defined this class of compounds as “exogenous substances that cause adverse health effects in an intact organism, or its progeny, consequent to changes in endocrine function” [1]. Emerging evidence from wildlife and laboratory studies indicates that some synthetic chemicals may interfere with the endocrine system in a broad variety of ways, including