参芪苈心方对心肌细胞能量代谢的影响.pdfVIP

中国科技论文在线 参芪苈心方对心肌细胞能量代谢的影响# 胡鸣旭，刘莉** （黑龙江中医药大学附属第一医院心内科，哈尔滨 150040） 5 摘要： 目的：观察参芪苈心方对去甲肾上腺素诱导H9C2 大鼠心肌细胞能量代谢的影响， 探讨参芪苈心方治疗心力衰竭的作用机制。方法：H9C2 大鼠心肌细胞分为正常对照组、模 型组、曲美他嗪组，参芪苈心组。后3 组分别加入正常大鼠血清10 μL、盐酸曲美他嗪含药 血清 10 μL、参芪苈心方含药血清 10 μL ，各组均加入去甲肾上腺素（最终浓度均为 2 μ mo1/L ），每组设8 个复孔,在加药后24 小时，48 小时进行指标检测及倒置显微镜观察，MTT 10 法测定细胞增殖率，高效液相色谱及闪烁荧光法检测细胞能量代谢情况。结果：参芪苈心方 能增加H9C2 细胞增殖活性，并通过抑制受损心肌细胞脂肪酸氧化，提高葡萄糖有氧氧化来 促进心肌细胞内ATP 的合成，并优于西药盐酸曲美他嗪组。结论：参芪苈心方能有效调节 受损心肌细胞能量代谢，这可能是参芪苈心方治疗充血性心力衰竭机制之一。 关键词：H9C2 心肌细胞；参芪苈心方；能量代谢；有氧氧化 15 中图分类号：R242 the effect of Shenqilixin Recipe on energy metabolism of cardiomyocytes Hu Mingxu, Liu Li 20 (No.1 hospital of heilongjiang university of TCM, ward of cardiology, Harbin 150040) Abstract: Objective: To observe the effect of Shenqilixin Recipe on the energy metabolism of H9C2 cells induced by norepinephrine , and explore the treatment mechanism of Shenqilixin Recipe on heart failure. Methods: H9C2 rat cardiomyocytes were divided into normal control group, model group, trimetazidine group and Shenqilixin group. The last three groups were added 25 with normal rat serum 10μL, serum with 10μL trimetazidine, serum with 10μL Shenqilixin Recipe and each group added norepinephrine (final concentration was 2μmo1 / L), each group containing eight wells. indicator tested and microscope observed at the 24 hours and 48 hours, cell proliferation rate determined by MTT, and cellular energy meta