104 个小麦品种(系) 脂肪氧化酶活性.pdfVIP

中国农业科学 2011,44(9): 1798-1805 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2011.09.005 104 个小麦品种（系）脂肪氧化酶活性 郑文寅，王 慧，崔文礼，张文明，姚大年 （安徽农业大学农学院，合肥230036 ） 摘要：【目的】研究小麦籽粒中的脂肪氧化酶（LOX）活性的变异规律及分布特点，为从遗传育种角度改善小 麦籽粒的耐储藏品质提供依据。【方法】选用104 个小麦品种（系）为试验材料，用分光光度计法测定其LOX 活性。 【结果】2 年测定的供试小麦品种（系）间的LOX 活性均达到极显著差异，变异系数均大于30%，年份间LOX 活性 相关极显著（r=0.605），表明2 个年度的品种 （系）间差异顺序基本一致，认为LOX 活性主要受遗传控制。在上 述分析基础上,用最长距离法将供试的104 份材料的LOX 活性聚为5 类。【结论】供试小麦品种（系）间LOX 活性 差异显著；在供试的104 个小麦品种（系）中，筛选到6 份低LOX 活性的品种资源，且多为中-强筋白皮小麦。 关键词：小麦；品种；脂肪氧化酶；活性；变异 Lipoxygenase Activity in 104 Wheat Varieties ZHENG Wen-yin, WANG Hui, CUI Wen-li, ZHANG Wen-ming, YAO Da-nian （Department of Agronomy, Anhui Agricultural University, Hefei 230036) Abstract: 【Objective 】 Grains lipoxygenase (LOX) activity is closely related to the storage quality of wheat (Triticum aestivum L.). Studies on the variation and distribution of grain LOX activity in wheat could provide a basis for genetic improvement of the storage quality.【Method 】The LOX activity was determined spectrophotometrically in 104 wheat varieties.【Result 】The results showed that the variation of LOX activity of the experimental varieties in two crop seasons was significant and the coefficients of variation were all more than 30.00%, also there were highly significant correlation between LOX activity of two crop seasons (r=0.605) ，which indicated that LOX activity was mainly controlled by gene. Based on longest distance (Euclidean distance) method, all the varieties could be clustered into 5 major groups. 【Conclusion 】Variation of LOX activity in the experimental wheat varieties is significant, and six varietal resources with lower LOX activity were selected from the ex