家兔眼球后注射替曲朵辛的麻醉效果及毒副作用研究.pdfVIP

维普资讯 眼科研究2004年12月第22卷第6塑 617 · 实 验 研 究 · 家兔眼球后注射替曲朵辛的麻醉效果 及毒副作用研究 刘瑜玲 张 强 杨艳芳 Anestheticandsideeffectsfollowingretrobulbarinjectionof Tetr0d0t0xin inrabbit LiuYuling，ZhangQiang，rangYanfang．DepartmentofClphthalmology，ThirdHospital ofPekingUniversity，Beijing100083，China Abstract Objective TodeterminetheanestheticeffectivenessofTetrodotoxin(rTx)anditstoxicitytoretinaafterits retrobulbarinjection． Methods Healthrabbitswereassignedtosixgroupsand6rabbitsforeachgroup．50 LofTTx(0．5 g， 1．0 g，2．5 g，5 gor10 g)wereinjectedretrobulbarlyintotherighteyesofrabbitsin5experimentalgroupsrespectivelyand 5LLLof2％ lidocainefortheothergroup．and thelefteyeofeach rabbitreceived50trLofpH 4．3sodium citratevehicleasa contro1．Cornealsensation wastestedafteradministrationofdrugs．Slit—lampexamination at12and24hoursafteradministration， electroretinography(ERG)beforeand24hoursafteradministrationandlightelectronmicroscopyexaminationafter1weekwere performedtodetectdrugtoxicitytooculartissues． Results A long—actingtopicalanestheticeffectwasdemonstratedatadoseof 5 gTTX，andthelasting—timewasfrom 0．37to5．7hoursin0．5to5I,LgofTTX．Shorter—actinganestheticactionupto0．34hours wasofundinlidocaine—injectedrabbits．10 gof1vrxresultedinrabbitdeathduetosystemictoxicity．DecreaseofERGb-waveand morphologicchangeswereseen in5．0 gTTX group and nothese changesin2．5 g IX group． Conclusion IX isall effective，long—lastingtopicalanestheticagentofrocularpaincontrol，andless2．5 gofrTX isasafedosetotopicalapplication． Keywords tetrodotoxin；retrobulbarinjection；toxicity 摘要 目的 对替曲朵辛 (TTX)溶液球后注射的麻醉效应、安全性和视网膜毒副作用进行初步评价。 方法 健康 成年家兔随机分组 ，每组6只，其中有眼球后注射不同剂量 TTX及利多卡因溶液，左眼给予等量溶剂对照 (柠檬酸钠缓 冲液 ，pH4．3)；分别采用角膜感觉试验、裂隙灯、视网膜电图、光