SD大鼠不同肠缺血时间观察长期存活率模型的筛选.pdfVIP

31:MEK学杂,x-2007年M23查盗19塑 2983 SD大鼠不同肠缺血时间观察长期存活率模型的筛选 黄品婕 蔡 19 甘小亮 沈 宁 黑子清 摘 要 目的探讨SD大鼠肠缺血合适缺血时间筛选适合于观察肠缺血再灌注长期存活率的SD大鼠肠缺 血再灌注模型。方法:采用肠系膜上动脉夹闭法建立小肠缺血再灌注模型，将60只sD大鼠随机分为5组，A组为 假手术组，B一E组分别为肠缺血60,75,90,120min组，每组12只。术后正常饲养，观察大鼠1一7d的存活率和 存活状态。结果:缺血60min组存活率及动物状态与假手术组相比无明显差异(P0.05)0缺血75min组第1天存 活50%,第3,7天均存活42%，动物毛发尚有光泽，反应灵歌，与假手术组((100%)相比，存活率差异有统计学意义 (PO}05)缺血90min组和缺血120min组第1,3,7天存活率明显低于假手术组(P0.01)，动物毛发无光泽，低 头、躬背，反应441,活动减少。缺血75min组第3天存活率及缺血90min,缺血120min两组的存活率明显低于 缺血60min组(P0-05)e结论:随小肠缺血时间延长，再灌注大鼠术后存活率下降。肠缺血75minSD大鼠是肠缺 血再灌注观察长期存活率的模型相对理想的选择。 关键词 再灌注损伤 存活率 大鼠 肠缺血 Durationofintestinalischemiainratsandscreeningofthemarinemodeloflong-termsurvivalHUANGPinjie, CAIfun,GANXiao-liang,SHENNing,HEIZi-grog.DepartmentofAnesthestofogy,ThirdAffiliatedHospital,SunYat- SenUnivers妙，Guangzhou510630,China Correspondingauthor:HEIZi-gingE-mad;heiz匆ing@sina [Abstract]ObjectiveToexplorethepropertimeofsmallintestinalischemiainSDratsandtoscreenthemurine modelofintestinalischemia/reperfusion(I/R)suitableforobservationoflong-ternsurvival.MethodsThemunne modelofintestinalI/Rwasconstructed切clampingthesuperiormesentericartery.60SDratswererandomlydividedinto 5groups:sham-operationgroup(groupA)andgroupsRtoEwithintestinalischemicdurationof60,75,90,and120 miniles,respectively(12foreachgroup).Theratswerefedaftertheprocedureandtheirsurvivalstateandsurvivalrate wereobservedin7days.ResultsThesurvivalstateandsurvivalratedidnotdiffersignificantlybetweengroupAand groupB (P0.05).TherewasstatisticaldifferenceinsurvivalratebetweengroupCandgroupAwithasurvivalrateof 5Wc ondayIand42%ondays3and7inthepreviousgroup (P0.05).Thesurvivalratesweremarkedlylowerin groupsDandEthanthatingro