香稻品种遗传多样性研究.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2006 ,34(22) :5794 - 5797 责任编辑 刘月娟 责任校对 孙能森 香稻品种遗传多样性研究 1 ,2 3 2 2 1,2 * 1,2 * 陈远孟 , 张向军 , 陈传华, 杨新庆 , 李杨瑞 , 李容柏 ( 1. 广西大学农学院, 广西南宁530004 ;2 . 广西农业 科学院水稻研究所, 广西南宁530007 ;3 . 广西农业科学院广西作物遗传改 良生物技术重点实验室, 广西南宁530007) 摘要 利用SSR 分子标记分析78 份外引香稻 品种、18 份在广西种植 的香稻 品种 以及24 份具有代表性的非香的广西地 方栽培稻种质资 源的遗传多样性, 检测到100 条多态性片段, 每个 引物可检测到3 ～14 个多态性片段, 平均为6 .25 个 。聚类结果表 明: 利用16 个SSR 标 记可 以明显地把香稻 与非香稻 品种聚类为2 大类; 在78 份外 引香稻种质 中, 在遗传距 离分别为0 .64 和0 .56 处,43 份和 14 份各 自聚为一 类, 占73 .1% , 18 份 与广西种植 的香稻品种聚类, 占23 . 1% ,3 份与非香的栽培稻 品种聚为一类, 占3 .8 % 。不 同类组香稻 的Nei ’s 遗传距 离估算表 明, 传统的南亚香稻 的遗传多样性显著大于改 良的南亚香稻, 改 良的南亚香稻 的多样性又显著大于广西当前种植 的香稻种质 和广西代表性的非香稻种质 。 关键词 香稻;SSR ; 聚类分析; 遗传距 离 中图分类号 S511 文献标识码 A 文章编号 0517 - 6611(2006) 22 - 5794 - 04 Study on Genetic Diversity in Aromatic Rice CHEN Yuan- meng et al ( College of Agriculture ,Guangxi University ,Nanning ,Guangxi 530004) Abstract Genetic diversity of 78 accessions of aromatic rice varieties was analyzed and compared with 24 accessions of non- aromatic rice with SSR mark- ers . Among the rice materials ,78 aromatic rice were derived fromabroad and 18 aromatic rice were planted in Guangxi province , while 24 non- aromatic rice were the representative samples of Guangxi native rice germplasm.Atotal of 100 polymorphic bands were present amongthe rice varieties and an aver- age number of alleles per SSRlocus was 6 .25 with a range from3 to 14 . Results of analysis with 16 SSR markers showed aromatic and non- aromatic vari- eties were basically clusteredi nto two groups . Amongthe 78 aromatic varieties derived fro mabroad ,73 .1 % were clusteredi nto group ,23 .1 % clustered to- gether with 14 Guangxi aromatic varieties , whereas only 3 .8 % c