化疗对晚期食管癌患者细胞免疫及调节T细胞的影响.docVIP

2014-1-25主任加工版 化疗对晚期食管癌患者细胞免疫及调节T细胞的影响 廖 娟，周云峰 （川北医学院附属医院，四川 南充 637000） [摘要]目的：探讨晚期食管癌患者化疗前后细胞免疫及调节T细胞（tregs）的变化及其临床价值。方法：采用流式细胞术检测健康志愿者及食管癌患者化疗前后外周血CD4+T、CD8+T细胞及调节T细胞的比例变化。结果：晚期食管癌患者化疗前外周血CD4+T细胞所占比例和CD4+T/CD8+T均较化疗后低，且二者均低于健康对照组（P＜0.05）。晚期食管癌患者CD4+CD25+T细胞和Tregs细胞所占比例化疗后较化疗前有所降低，二者均明显高于健康对照组。结论：化疗能明显减轻晚期食管癌患者的肿瘤免疫耐受，改善免疫状况。 [关键词]免疫调节T细胞；食管癌；化疗；细胞免疫 [中图分类号]R [文献标志码]A doi:10.11851/j.issn.1673-1557.2014.01.0 Effect of Chemotherapy on Cellular Immune Function and Tregs in Patients with Advanced Esophageal Cancer Liao Juan,Zhou Yunfeng （North Sichuan Medical College Affiliated Hospital,Sichuan Nanchong 637000,China） [Abstract]Objective:To investigate the changes of cellular immune function and CD4+CD25+ regulatory T cells (Tregs) in peripheral blood of patients with advanced esophageal cancer before and after chemotherapy and their clinical implications. Methods:The proportion of CD4+CD25+Tregs among CD4+T lymphocytes and the proportion of CD4+ and CD8+T cells of PBMC in peripheral blood of 32 patients with advanced esophageal cancer were determined by flow cytometry before and after chemotherapy.Results:Compared with control group,the levels of CD4+T lymphocytes and CD4+/CD8+ T lymphocytes were decreased in patients with advanced esophageal cancer before and after chemotherapy,but the data after treatment was higher than that before. The percentage of CD4+CD25+T cells and Tregs among CD4+T lymphocytes significantly changed after chemotherapy as compared with that before chemotherapy, the difference was significant between the patients and the healthy people .Conclusion: chemotherapy can improve the immune function of patients with advanced esophageal cancer. [Key words]immunoregulatory cells;esophageal cancer;chemotherapy;cellular immunity 食管癌是我国发病率较高的恶性肿瘤之一，总体5年生存率较低。与其他肿瘤一样，食管癌的发生发展与肿瘤免疫耐受明显相关，调节T细胞作为主要的免疫负向调节细胞促进肿瘤的免疫耐受，从而促进肿瘤的发生发展。化疗作为临床治疗食管癌的重要方法之一，尽管已广泛应用于临床，但能否影响食管癌患者调节T细胞，目前尚不清。本研究从肿瘤免疫角度，初步探讨化疗对晚期食管癌患者调节T细胞及细胞免疫功能的影响，为食管癌治疗