比较MSP法与PS法检测胃癌组织中Runx3启动子甲基化.pdfVIP

24 Journal of Medical Science in Central South China,January2012,Vol.40,No.1 文章编号:2095-1116(2012)01-0024-07 ·基础医学· 比较 MSP法与PS法检测胃癌组织中Runx3启动子甲基化 唐国华,孙少卫 (南华大学 生命科学研究中心,湖南衡阳421001) 摘摇 要:摇 目的摇 探讨甲基化特异性聚合酶链法(MSP)和焦磷酸测序法(PS) 检测Runx 3基因甲基化的特异 性。 摇 方法摇 选取150例胃癌患者[依据美国癌症联合委员会(AJCC)分期标准,分肿瘤的早期,进展期两组]胃切除 的胃癌标本及50例无癌变胃黏膜标本。 分别以MSP法和PS 法同时检测其Runx3启动子甲基化程度。 摇 结果摇 胃 癌组织与正常胃黏膜相比,其Runx3基因甲基化程度的差异均有显著性(MSP 法:67.3%,40.0%,P =0郾002;PS 法: 76郾0%,30.0%,P0.001)。 MSP法显示:Runx3 甲基化与肿瘤大小相关(P0.05);而PS法分析显示:进展期胃癌的 Runx3基因甲基化率高于早期胃癌(P0.05);且其甲基化状态与肿瘤的大小(P =0.004)、Lauren分级(P =0郾043)、 浸润的程度(P0.001)、淋巴结转移(P=0.021)及肿瘤TNM临床分期(P=0.045)等临床病理特征之间存在相关性。 结论摇 PS法与MSP法相比,敏感性更高,PS检测结果与临床病理学结果之间存在显著相关性。 关键词:摇 甲基化;摇 Runx3基因;摇 胃癌;摇 PS法;摇 MSP 法 中图分类号:R735鄄2摇 摇 摇 文献标识码:A Comparison of MSP and PS in Detection of Runx3 Gene Methylatio TANG Guo鄄hua,SUN Shao鄄wei (Life Science Research Center,University of South China,Hengyang,Hunan421001,China) Abstract:摇 Objective摇 To investigate the specificity of MSP and PS in detection of Runx3 gene methylation.摇 Methods摇 We selected 150 specimen from patients with gastric canner and 50 normal specimen in our experiment,and used MSP and ps tests to detect the methylation status of Runx3 gene promoter.摇 Results摇 Compared to normal tissue, there are dramatic difference in methylation status of Runx3 gene CpG island in human gastric cancer(MSP:67.3 vs 40郾0%,P=0.002;ps:76.0 vs30.0,P0.001).MSP test demonstrated that Runx3 gene methylation was only related to tumor size(P0.05).Pstest showedthattheextent of Runx3 genemethylationwashigher inadvancedgastriccancerthan in early gastric cancer,and the extent of methylation status of Runx3 gene was related to tumor size(P =0.04)