杂交瘤细胞代谢流分析模型及其在批式培养中的应用.pdfVIP

第 20 卷第 1 期 无 锡 轻 工 大 学 学 报 Vol. 20 ,No. 1 　 　 　 　　　　 　 2001 年 1 月 Journal of Wuxi University of Light Industry 　 　 　 　　　　 Jan. ,2001 ( ) 文章编号 :1009 - 038X 2001 01 - 0011 - 05 　 杂交瘤细胞代谢流分析模型及其在批式培养中的应用 仝志明 , 　曹竹安 (清华大学化学工程系 ,北京 100084) 摘　要 : 通过物料衡算和线性规划方法建立了进行代谢流分析的数学模型 ,并首次应用于批式培 养过程. 分析结果表明 ,细胞对葡萄糖等底物的代谢效率很低 ,细胞对谷氨酰胺等氨基酸浓度的变 化响应迟缓 ,在底物充裕的迟滞期及谷氨酰胺限制条件下的代谢效率高于对数生长期. 另外限制 谷氨酰胺等氨基酸进入能量代谢 ,既可减少氨的积累 ,也会减少乳酸的生成. 关键词 : 杂交瘤 ;代谢流分析 ; 物料衡算 ; 批式培养 中图分类号:Q813 　　　　　　　　文献标识码 : A Metabolic Flux Analysis Model of Hybridoma Cell and Its Application to Batch Culture TON G Zhiming , CAO Zhuan (Department of Chemical Engineering , Tsinghua University ,Beijin g 100084 , China) Abstract : An improved mathematical model of metabolic flux analysis by material balances and linear pro gramming method was established , which was first applied to batch culture process in this paper. The analysis results indicate that the metabolic efficiency of substrates such as glucose is very low. It showed that the response of cell metabolism to the step change of glutamine and other amino acid con centration was timedelayed. Metabolic efficiency was higher both in lag phase with highest substrate concentrations and glutaminelimited phase than that in exponential phase. It was also concluded that restraining glutamine from entering energy metabolism could reduce the accumulation of both ammonia and lactate. Key words : hybridoma ; metabolic flux analysis ; material balances ; batch culture 　　代谢流的分析在微生物培养中应用比较广