酶催化动力学光度法测定镉(Ⅱ).pdfVIP

《化学通报》在线预览版 酶催化动力学光度法测定镉(Ⅱ) 柳畅先 梁曦 何进星 (中南民族大学化学与材料科学学院 分析化学国家民委重点实验室 武汉 430074) 摘要 基于醇脱氢酶(ADH)催化乙醇生成乙醛的反应中镉离子对于酶的抑制作用原理,通过测定还原型烟酰胺 腺嘌呤二核苷酸(NADH)吸光度的变化率得出其酶促反应速度, 对应于镉离子浓度而制得标准曲线。检出限为 0.02μmol/L。讨论了pH、温度和共存离子对测定的影响。本方法具有快速、灵敏、干扰少的优点。 关键词 镉(Ⅱ) 酶法测定 动力学光度法 醇脱氢酶 Determination of Cadmium( Ⅱ) by Enzymatic Kinetic Spectrophotometry * Liu Changxian Liang Xi He Jinxing (Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry, South-Central University for Nationalities, Wuhan 430074) Abstract An enzymatic method was applied to determine cadmium( Ⅱ) based on the inhibition of cadmium( Ⅱ) for the catalytic reaction of alcohol to form acetaldehyde by alcohol dehydrogenase(ADH) . The rate of the absorbance change of nicotinamide adenine dinucleotide reduced form(NADH) was determined for calculating the velocity of the enzymatic reaction and calibration curve of cadmium( Ⅱ) was prepared. The detection limit was 0.02 μmol/L. The effects of the pH, temperature and the coexisted ions on the determination were discussed. The method is rapid, sensitive and less interference. Key words Cadmium( Ⅱ), Enzymatic determination, Kinetic spectrophotometry, Alcohol dehydrogenase 镉属于剧毒物质，在自然环境中含量很低, 但可以通过食物链富集后达到相当高的浓度， 对人体健康产生不良影响。因此,其含量是环境、生物、食品试样检测的重要指标。测定镉的方 ［1］ 法主要有原子吸收分光光度法、双硫腙分光光度法和阳极溶出伏安法 。分光光度法是测定金 属离子最常用的方法，但灵敏度较低，原子吸收分光光度法和阳极溶出伏安法测定的灵敏度很 高，但其仪器相对昂贵，且原子吸收分光光度法需克服由于火焰气体背景、化学反应以及电离 引起的干扰，阳极溶出伏安法的操作也较复杂。近年来酶法在分析测定方面的应用研究很活跃， ［2～6］ 该方法可测定在酶促反应中作为底物、辅酶、激活剂和抑制剂而存在的各种物质