动脉粥样硬化易损斑块组织荧光团分析及其多模态早期诊断技.pdfVIP

Vol. 4 No.1/ Jan.Vol. 4 No.1/ Jan. 010010 动脉粥样硬化易损斑块组织荧光团分 析及其多模态早期诊断技术 Fluorophore analyses of vulnerable atherosclerotic plaque and its early detection based on multimode imaging techniques 沈洋　崔晗　刘新　郑海荣 摘 要 本文简要介绍了基于时间分辨激光诱导荧光光谱技术的动脉粥样硬化斑块诊断方法，并详细讨论了血管壁组织荧 光团与激发荧光波长及湮灭时间(荧光寿命)之间的关系。存在于血管壁的组织荧光团主要有弹力素、胶原蛋白(I型和III型)以 及脂质成分(游离胆固醇、胆甾醇酯、低密度脂蛋白等)。弹力素具有较宽的激发光谱带，其主要存在于正常动脉；而胶原 具有窄的光谱带和蓝移峰谱，并具有最长的荧光寿命，是纤维性斑块的主要荧光团成分；脂质成分具有最短的荧光寿命， 是不稳定性斑块即脂质性斑块的主要荧光团。本文最后还介绍了一种具有互补特性的多模态动脉粥样硬化易损斑块诊断系 统。 关键词 动脉粥样硬化；荧光团；时间分辨激光诱导荧光光谱；血管内超声；多模态诊断 ABSTRACT The diagnosis of atherosclerotic plaque based on time-resolved laser-induced fluorescence spectroscopy and the relationship of the endogenous fluorophores in lesions with the wavelength-resolved and especially the time- resolved data were investigated. The major fluorophores in arterial tissues are elastin and collagen (type I and III), and lipid components such as free cholesterol, cholesterol esters and LDLs. Elastin which has broad spectral emission is the primary fluorophore in normal artery, whereas collagen which has narrow band and blue-shifted peak emission and the highest lifetime is the mainly fluorophore in collagen-rich plaques. Lipid components that have the fastest fluorescence dynamics mainly exist in lipid-rich lesions which are always instability. At last a dual-modality system combined two complementary techniques was also introduced to diagnose vulnerable plaques. KEYWORDS　atherosclerosis;fluorophore;time-resolved laser-induced fluorescence spectroscopy;intravascular ultrasound;hybrid diagnosis 1 Introduction umerous studies [1] have shown that Acute Coronary Angiography (CAG), Coronary Angio