技术方法四种消毒方式对简易呼吸囊的灭菌效果评价.pdfVIP

J South Med Univ, 2013, 33(7): 1067-1070 doi 10.3969/j.issn. 1673-4254.2013.07.27 ·1067 · 技术方法 四种消毒方式对简易呼吸囊的灭菌效果评价四种消毒方式对简易呼吸囊的灭菌效果评价 1 2 3 1 1 陆爱武 ，王 媚 ，聂志强 ，郭远波 ，王春晓 1 2 3 广东省人民医院 麻醉科，细菌室，心外科流行病研究室，广东 广州 510080 摘要：目的研究观察4 种不同消毒方式对简易呼吸囊的消毒效果和微生物残留状况，探讨加强呼吸囊消毒方式方法管理的必要 性。方法分4 组消毒试验：G1组43 例，500 mg/L 二氧化氯（ClO2 ）喷雾；G2 组28 例，酒精喷雾；G3 组47 例，50 mg/L 三氯异氰尿 酸（TCCA）浸泡；G4 组46 例，50 mg/L ClO2 溶液浸泡。各组于消毒后30 min 取样接种培养，菌落计数后用卡方秩和进行统计学 分析，并对残留菌鉴定分型。结果（1）4 组菌落计数有显著性差异（P0.001）。细菌残留率：G1组为0% ，G2 组53.6% ，G3 组 27.7% ，G4 组21.7% ，各组间比较统计学有显著性差异（P0.001）。（2）残留菌鉴定结果显示铜绿假单胞杆菌和饱曼不动杆菌，溶 血性葡萄球菌等常见耐药性致病菌。结论 麻醉简易呼吸囊以500 mg/L ClO2 喷雾方式消毒消果最好。中低效TCCA 反复浸泡 长期使用，可能导致耐药致病菌生长。 关键词：简易呼吸囊；消毒；二氧化氯；三氯异氰尿酸；耐药致病菌 Sterilizing effect and residual bacteria of four different methods for sterilizing simple breathing vesicles 1 2 3 1 1 LU Aiwu , WANG Mei , NIE Zhiqiang , GUO Yuanbo , WANG Chunxiao 1Department of Anesthesiology, 2Department of Bacteriology, 3Department of Cardiac Surgery and Epidemiology, Guangdong Province Peoples Hospital, Guangzhou 510080, China Abstract: Objective To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial residue. Methods The disinfection tests were divided into 4 groups: G1 group (43 cases) with 500 mg/L chlorine dioxide spray, G2 group (28 cases) with alcohol spray, G3 group (47 cases) with 50 mg/L trichloro isocyanuric acid (TCCA) immersion, and G4 group (46 cases) with 50 mg/L chlorine dioxide solution immersion. Afrer 30 min of dsinfection, each group was examined by bacterial culture and colony count. The residual bacteria were identified and typed. Results The 4 methods showed significant differences