血清与组织glactin-3联合检测在甲状腺癌早期诊断中的价值.pdfVIP

J South Med Univ, 2013, 33(7): 1027-1030 doi 10.3969/j.issn. 1673-4254.2013.07.18 ·1027 · 临床研究 血清与组织血清与组织glactin-glactin-33 联合检测在甲状腺癌早期诊断中的价值联合检测在甲状腺癌早期诊断中的价值 1，2 2 1 1 1 1 2 薛 刚 ，刘军超 ，黄 静 ，张 静 ，张文静 ，吴靖芳 ，尚小领 1 2 河北北方学院 组织胚胎学教研室，附属第一医院耳鼻喉科，河北 张家口 075000 摘要：目的探讨甲状腺癌患者血清与组织中半乳凝素-3（galectin-3, gal-3）的表达水平及意义。方法 收集河北北方学院附属第 一医院就诊甲状腺肿瘤患者159例，正常对照组16例。采用酶联免疫吸附法( ELISA)检测血清gal-3 浓度，免疫组织化学SP 和 原位杂交技术法检测甲状腺手术标本中gal-3 蛋白和mRNA 的表达。结果 Gal-3 蛋白和mRNA 均表达于胞浆，呈棕黄色颗粒。 66 例乳头状癌gal-3 表达阳性率96.97% ；6 例滤泡癌阳性率83.33% ；36 例乳头状增生阳性率16.67% ；51 例腺瘤gal-3 阳性率 11.74% ；Gal-3mRNA 阳性率略低于其蛋白，且与蛋白表达呈正相关。甲状腺癌组血清gal-3 浓度明显高于良性乳头状增生组、 腺瘤组和正常对照组（均为P0.001）。血清gal-3浓度在甲状腺癌组织阳性组明显高于阴性组P0.05。结论 检测血清gal-3浓 度及组织表达可以提高甲状腺癌的诊断率。 关键词：半乳糖血凝素-3 ；酶联免疫吸附试验；免疫组化；原位杂交；甲状腺癌 Detection of glactin-3 in both serum and tissue for early diagnosis of thyroid carcinoma 1, 2 2 1 1 1 1 2 XUE Gang , LIU Junchao , HUANG Jing , ZHANG Jing , ZHANG Wenjing , WU Jingfang , SHANG Xiaoling 1 2 Department of Histology and Embryology , Department of Otorhinolaryngology, Head and Neck Surgery, First Affliated Hospital , Hebei North University, Zhangjiakou 075000, China Abstract: Objective To investigate the levels of galectin-3 in the serum and surgical specimens from patients with malignant and benign thyroid lesions and explore their clinical significance. Methods Serum samples were collected from 159 patients with thyroid neoplasms and 16 normal subjects for detection of galectin-3 level using ELISA. The expressions of galectin-3 protein and mRNA were also detected in the surgical specimens by immunohistochemistry and in sit