拉曼光谱在植物细胞壁研究中的进展分析讲解.docVIP

  拉曼光谱在植物细胞壁研究中的进展# 马静1，马建锋1，张逊1，许凤1,2** （1. 北京林业大学材料科学与技术学院，北京 100083； 5 10 15 20 25 30 35 40 2. 齐齐哈尔大学轻工纺织学院，黑龙江 齐齐哈尔 161006） 摘要：在能源和生态环境制约下，农林生物质替代化石资源生产生物燃料、化学品及生物基 材料已成为国内外许多学者研究的热点。全面了解农林生物质原料的化学组成及其结构特性 是高效利用农林生物质的基础。作为一种无损的检测技术，现代拉曼光谱能够在原位状态下 提供植物细胞壁区域化学和结构特性信息。本文简述了拉曼光谱技术原理，概括了拉曼光谱 在植物细胞壁主要组分的结构分析、纤维素和木质素的微区分布及其分子排列等方面的研究 进展，以促进该技术在植物细胞壁研究中的应用。 关键词：拉曼光谱；纤维素；木质素；微区分布；分子排列 中图分类号：TS01 Application of the Raman spectroscopy to the study of plant cell walls MA Jing1, MA Jianfeng1, ZHANG Xun1, XU Feng1,2 (1. College of Material Science and Technology, Beijing Forestry University, Beijing 100083; 2. College of Light Chemistry and Textile Engineering, Qiqihar University, Heilongjiang Qiqihar 161006) Abstract: Because of the inadequate supply of energy coupled with negative environmental impacts, lots of scholars pay more attention to the replacement of fossil resources with agriculture and forestry biomass for the production of bio-fuels, chemicals and biomaterials on a global scale. High-effective utilization of agriculture and forestry biomass is dependent on full understanding of their chemical composition and structural characteristics. A state-of-the-art Raman spectroscopy has evolved an important and nondestructive technique for plant research as information concerning histochemistry and structural characteristics of plant cell walls can be investigated in a close to native state. The principle of Raman spectroscopy was introduced briefly. The research progress in structural analysis of major components, micro-area distribution and molecular organization of the cellulose and lignin in the plant cell walls by Raman spectroscopy was summarized. The aim of the review is to promote the application of Raman spectroscopy to the study of plant cell walls. Keywords: Raman spectroscopy; cellulose; lignin; micro-area distribution; molecular organization 0 引言 拉曼光谱又称拉曼效应，是研究分子振动和转动的一种光谱方法。由于拉曼光谱具有样 品制备无特殊要求，可检测微克级样品，在样品原位进行成分和结构分析等优点，能快速、 简单、可重复、且无损的获得分子、原子水平上的样品信息。目