纳米氧化镍对3种绿藻的毒性效应.pdfVIP

中国环境科学 2013,33(10) ：1842~1849 China Environmental Science 纳米氧化镍对 3 种绿藻的毒性效应 雷静静,冯 佳,谢树莲* ( 山西大学生命科学学院, 山西 太原 030006) 摘要：通过配制不同浓度 (0,60,120,180,240,300mg/L) 纳米氧化镍(nNiO)悬浊液 ,对四尾栅藻 (Scenedesmus quadricauda) 、普通小球藻 (Chlorella vulgaris)和羊角月牙藻(Selenastrum capricornutum)进行连续胁迫 10d 的毒性试验,测定 nNiO 对 3 种绿藻生长状况、叶绿素 a、蛋 白质、脂质过氧化物丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的影响.结果表明,nNiO 胁迫对四尾栅藻、普通小球藻和羊 角月牙藻的生长均有一定抑制作用,在同一时间段内,总体呈明显剂量效应;nNiO 低浓度(60~120mg/L)胁迫对供试藻的影响表现为“抑制— 促 —抑制”,在一定时间内对供试藻有促进生长的作用;nNiO 胁迫对四尾栅藻、普通小球藻和羊角月牙藻的毒性效应表现为叶绿素 a 和蛋 白质含量减少,MDA 含量升高,抗氧化活性降低;羊角月牙藻对 nNiO 的耐受性较强, 四尾栅藻和普通小球藻较敏感. 关键词：纳米氧化镍；四尾栅藻；普通小球藻；羊角月牙藻；毒性效应；防御机制 中图分类号：X171.5 文献标识码：A 文章编号：1000-6923(2013)10-1842-08 * Toxic effects of nNiO on three species of green algae. LEI Jing-jing, FENG Jia, XIE Shu-lian (School of Life Science, Shanxi University, Shanxi, Taiyuan 030006, China). China Environmental Science, 2013,33(10)：1842~1849 Abstract ：The toxic effects were investigated on three green algae (Scenedesmus quadricauda, Chlorella vulgaris and Selenastrum capricornutum) for 10days by using nNiO suspensions in different concentrations (0, 60, 120, 180, 240, 300mg/L). The cell density, the contents of chlorophyll a, protein, malonyldialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) were measured respectively. The results indicated that: The growth of three species were inhibited under the stress of nNiO, and showed obvious dose effect during the same period; Effects of lower nNiO concentrations (60~120mg/L) on tested algae revealed a process of “inhibition-promotion-inhibition”, which meant that the growth of these algae was accelerated only in a certain period; Toxic effects of nNiO on three species demonstrated that the chlorophyll a and protein contents were d