急性髓系白血病细胞Flt-3表达及Flt-3-ITD突变分析.DOCVIP

临床医学论文-急性髓系白血病细胞Flt-3表达及Flt-3/ITD突变分析 ???????????????? 作者：汪亚伦，王彤，许凤，冮岩，王杰 【摘要】? 本研究探讨急性白血病（acute leukemia，AL）细胞株Flt-3基因表达和Flt-3/ITD突变与急性白血病特别是急性髓系白血病(acute myeloid leukemia，AML)发病的关系。采用RT-PCR分析联合测序的方法检测82例白血病细胞株，其中包括20例AML细胞株和57例急性淋巴细胞白血病细胞株（ALL），5株CML细胞株的Flt-3表达和Flt-3/ITD突变。结果表明： 77例AL细胞株中有48例Flt-3表达阳性，阳性表达率为62%，其中20例AML细胞株中有12例阳性，阳性表达率为60%；57例ALL细胞株中有33例阳性，阳性表达率为58%。5例CML细胞株中有3例阳性，阳性表达率为60%。12例Flt-3表达阳性的AML中有1例AMOL细胞株存在有异常表达（阳性率为8.3%），测序显示存在有29 bp的两个编码重复序列，其余Flt-3表达阳性的细胞株未检出重复序列。未分化B细胞系Flt-3基因表达阳性率明显高于成熟B细胞ALL (P0.05)。结论: Flt-3基因在不同种类的白血病细胞中存在着不同程度的表达，在其中1例AML中发现有Flt-3/ITD重复序列。Flt-3基因及Flt-3/ITD突变检测可能有助于ALL特别是AML的诊断。 【关键词】? Flt-3 基因 　　Analysis of Flt-3 Expression and Flt-3/ITD Mutation in Acute Myeloid Leukemia Cells 　　Abstract This study was aimed to? explore the relationship between Flt-3 expression，Flt-3/ITD mutation in acute leukemia (AL) cell line and pathogenesis of? AL，especially AML. The Flt-3 expression and? Flt-3/ITD mutation were detected by RT-PCR and sequencing method in 82 leukemia cell lines? including 20 AML，57? ALL and 5 CML cell lines. The results? indicated that? positive results of Flt-3 expression were obtained in 48? out of 77? AL cell line，the positive rate was 62%;? 12 cell lines were positive in 20? AML cell lines，the positive rate was 60%; 33 cell lines was?? positive in 57 ALL cell lines，the positive rate was 58%; 3 cell lines were? positive in 5 CML cell lines，the positive rate was 60%. There was abnormal gene product in 1 AMOL cell line out of 12 AML cell lines with? Flt-3 positive expression (positive rate 8.3%). DNA sequencing of abnormal gene product showed?two coding duplication sequence with 29 bp long. The positive rate of牋 Flt-3 expression in undifferentiated cell line was prominently higher than that in?mature B cell ALL (P0.05). It is concluded? that the Flt-3 expression is? different in various? leukemia cells. Flt-3/ITD duplication was found in one AML cell line. The detection of Flt-3 gene and Flt-3/ITD mutation may cont