器官移植受者微量CMV DNA与IL-10基因单核苷酸多态性的相关性研究.DOCVIP

临床医学论文-器官移植受者微量CMV DNA与IL-10基因单核苷酸多态性的相关性研究 ?????????????????? 作者：傅晶 郑建建 潘晓东 杨亦荣 陈必成 郑少玲? 【摘要】? 目的 研究白介素-10（IL-10）基因G1082A和C819T单核苷酸多态性（SNP）与移植术后外周血微量巨细胞病毒（CMV）DNA的关系。方法 采集176例移植术后受者，429例次的外周血。通过巢式聚合酶链反应（nPCR）技术检测微量CMV DNA。通过序列特异性聚合酶链反应（SSP-PCR）技术检测IL-10基因G1082A与C819T的变异情况。分析IL-10各基因型在微量CMV DNA受者中的分布频率，并对阳性组与阴性组进行比较。结果 研究人群中IL-10 G1082A和C819T基因型分布符合Hardy-Weinberg遗传平衡定律。CMV DNA阳性组IL-10 G1082A和C819T等位基因频率分别为G=3.75%、A=96.25%和C=32.50%、T=67.50%；CMV DNA阴性组分别为G=5.51%、A=94.49%和C=36.03%、T=63.97%。CMV DNA阳性组两SNP基因型及等位基因分布与其在CMV DNA阴性组中的分布差异无显著性（P0.05）。结论 目前尚不能认为IL-10基因G1082A与C819T单核苷酸多态性与移植受者微量CMV DNA阳性有相关性。 【关键词】? 白介素-10 单核苷酸多态性 移植 巨细胞病毒 　　【Abstract】? Objective? To investigate the relationship between the IL-10 G1082A and C819T s SNP and the trace CMV DNA of recipients. Methods?The trace CMV DNA in 429 samples of 176 patients was detected by nested polymerase chain reaction (nPCR). The IL-10 G1082A and C819Ts SNP were determined using sequence specific primers polymerase chain reaction (SSP-PCR). Results?In the 40 CMV DNA-positive samples, the G1082A allele frequencies were 3.75% for G and 96.25% for A, with the genotype frequencies 0% for G/G, 92.5% (n=37) for A/A, and 7.5% (n=3) for G/A; In the 136 CMV DNA-negative samples, the G1082A allele frequencies were 5.51% for G and 94.49% for A, with the genotype frequencies 0% for G/G, 88.97% (n=121) for A/A, and 11.03% (n=15) for G/A. In the CMV DNA-positive group, the C819T allele frequencies were 32.5% for C and 67.5% for T, with the genotype frequencies 5% (n=2) for C/C, 40% (n=16) for T/T, and 55% (n=22) for C/T; In the CMV DNA-negative group, the IL-10 C819T allele frequencies were 36.03% for C and 63.97% for T, with the genotype frequencies 11.76% (n=16) for C/C, 39.71% (n=54) for T/T, and 48.53% (n=66) for C/T. The two SNPs distribution of the frequencies were all statistically similar between the CMV DNA-positive/negative groups（P0.05）. Conclusions? The IL-10 gene G1082A and C819Ts SNP are not associated with the CMV