前列腺素E1对大鼠肝移植肾功能的保护作用.DOCVIP

临床医学论文-前列腺素E1对大鼠肝移植肾功能的保护作用 ??????????? 作者：阚彤 杨甲梅 严以群 吴孟超 【摘要】? 目的 探讨术中应用前列腺素E1(prostaglandin E1,PGE1)对大鼠肝移植肾功能的保护作用。方法 大鼠原位肝移植术中经颈内静脉灌注PGE1为治疗组，生理盐水和空白为对照组，观察术后1周存活率、1 h的尿量，测定血浆肌酐、尿素氮和肾组织中丙二醛(malondialdehyde,MDA)、谷胱甘肽(glutathione,GSH)含量，肾组织病理检查。结果 PGE1治疗组术后1 h尿量较对照组明显增加，肌酐和尿素氮水平均较对照组降低，PGE1治疗组肾组织中GSH含量显著高于两对照组，MDA含量低于两对照组。病理检查PGE1治疗组肾脏组织形态学损伤明显减轻。结论 术中应用PGE1能显著改善大鼠肝移植后的肾功能，其机制可能与对抗氧自由基损伤作用有关。 【关键词】? 前列腺素E1； 肾功能； 肝移植； 大鼠 　　Protective effect of prostaglandin E1 on renal function of rats after liver transplantation? 　??? 【Abstract】? Objective? To assess the protective effect of prostaglandin E1 (PGE1) on renal function of rats after liver transplantation. Methods? PGE1 treatment group was established by infusing PGE1 into internal jugular vein at 0.5 μg/(kg·min) during liver transplantation. Normal saline group and sham operation group were established as control. Oneweek survival rates were observed and urinary output 1 hour after liver reperfusion was recorded. Postoperative plasma creatinine (CRE) and urea nitrogen (UN) were determined every day during the first week. The levels of glutathione (GSH) and malondialdehyde (MDA) in kidney tissues were measured to assess ROI injury. Kidney tissue sections were stained with hematoxylin and eosin for histological examination. Results?Compared with the control groups, the urinary output 1 hour after liver transplantation in PGE1 group increased significantly, while the levels of CRE and UN decreased; the levels of GSH in kidney tissues in PGE1 group were significantly higher than those in control group, while the MDA was comparatively lower. Histological examination of kidney demonstrated beneficial effects of PGE1 on nephric tubule.?Conclusions?Application of PGE1 during operation can promote the renal function of rats after liver transplantation, which may be related to the resistance of injury from oxygen free radical. ??? 【Key words】? Prostaglandin E1;? Renal function;? Liver transplantation;? Rats ??? 肝移植术后肾功能损害是