利用ISSR 标记分析青麻种质资源遗传多样性.pdfVIP

中国农业科学 2008,41(11):3532-3541 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.11.012 利用ISSR标记分析青麻种质资源遗传多样性 白占兵，粟建光，陈基权，戴志刚，王凤敏，龚友才 （中国农业科学院麻类研究所，长沙 410205 ） 摘要：【目的】利用分子标记分析来自中国 11 个省（市）的 48 份青麻种质资源的遗传多样性，为青麻资源 利用和育种提供分子生物学依据。【方法】利用内部简单重复序列多态性（ISSR）分子标记检测青麻种质资源的遗 传多样性。【结果】在 48 份青麻种质资源中，9 条 ISSR 引物扩增出 82 条带，多态条带比率（PPB）为 88.89%,扩 增产物片段大小在0.1～3.0 kb。基于UPGMA聚类，将青麻分为3大类。【结论】中国青麻的遗传多样性水平较高， 实验结果支持青麻起源于中国。 关键词：青麻；种质资源；ISSR；遗传多样性；起源 Genetic Diversity of Velvetleaf Germplasm Revealed by ISSR Marker BAI Zhan-bing, SU Jian-guang, CHEN Ji-quan, DAI Zhi-gang, WANG Feng-min, GONG You-cai (Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205) Abstract: 【Objective 】This article analyzed the genetic diversity of 48 velvetleaf from 11 provinces of China, and provided biological information on utilization and breeding of velvetleaf germplasm. 【Method 】Inter-simple Sequence Repeat (ISSR) markers were applied to detect genetic diversity of 48 velvetleaf germplasm in China. 【Result 】82 bands were amplified with nine ISSR primers, with 88.89% polymorphic ones. The amplified DNA fragments were 0.1-3.0 kb in size. The dendrogram was constructed by UPGMA method, showing 48 velvetleaf germplasm clustered into three groups. 【Conclusion 】There is a high genetic diversity in velvetleaf germplasm. The result supported the conclusion that China is the origin of velvetleaf. Key words: Velvetleaf; Germplasm; ISSR; Genetic diversity; Origin 集、保护、研究势在必行。目前中国共保存青麻种质 0 引言 资源311 份，其中中国农业科学院麻类研究所205 份， 【研究意义】青麻（Abutilon theophrasti Medicus ） 辽宁省经济作物研究所106 份，研究这些种质资源为 为锦葵科青麻属一年生韧皮纤维作物，又称苘麻、桐 利用这些材料提供了一定的理论意义。青麻抗逆性强、 麻、白麻等