婴幼儿先天性心脏病并心力衰竭肌浆网钙转运蛋白基因表达研究.pdfVIP

心力衰竭组较心功能正常组亦明显降低 (P0.05)，且RyRmRNA表 达水平与心力衰竭严重程度(Ross评分)呈负相关(二一0.36,P0.05). Cat+-ATP酶mRNA,RyRmRNA表达水平改变与年龄、性别、基础 、 心脏病 (房间隔缺损、室间隔缺损)等因素无关r结论:婴幼儿先 天性心脏病并心力衰竭时心肌细胞肌浆网Ca2+-ATP酶mRNA,RyR mRNA表达下调，提示除血液动力学改变外，心肌细胞肌浆网 Cat+-ATP酶、RyR基因表达与婴幼儿先天性心脏病并心力衰竭的发 生或发展有关。近年来，一系列发达国家有关心力衰竭发生机制的 动物实验和临床研究资料表明心肌细胞肌浆网钙转运蛋白基因表达 水平改变有可能为心力衰竭发生的关键因素，但有关婴幼儿先天性 心脏病合并心力衰竭时心肌细胞肌浆网CaZ+-ATP酶、RyR研究资料 极少。国内尚未见相关报道，有待进一步研究。 关健词: C扩十-ATP酶 罗纳丹受体 心力衰竭 先天性 心脏病 ALTERATIONSOFGENEEXPRESSIONOF CARDIACSARCOPLASMICRETICULUM PROTEINSININFANTSW ITH CONGENITALHEARTDEFECTAND CONGESTIVEHEARTFAILURE Postgraduate YinWei Tutor ShiShuhua ZhengXiaoqin Abstract Objective:ToinvestigatechangesofmRNAexpressionlevelsoftwo kindsofcardiacsarcoplasmicreticulum (SR)CaZ+transfer proteins-calcium-adenosin triphosphatase (C2_-ATP..) and ryanodinereceptor(RyR)ininfants(within3year-old)withcongenital heartdefect(CHD)andcongestiveheartfailure(CHF);Toexplorethe molecularmechanismsofCHFininfantswithCHDinordertoprovide theoreticalandexperimentalbasisforthetreatment.Methods:By reverse transcription-polymerase chain reaction (RT-PCR)and multimediatedgelimaginganalysissystem,wemeasuredexpression levelsofSRC2+-ATPwamRNAandRyRmRNA intherightatrial tissuerfomthreegroupswithCHF(groupA:moderatetosevereCHF; groupB:mildCHF;groupC:NoCHF).Allthepatientsunderwent cardiacsurgeryforventricularseptaldefect(VSD)andatrialseptal defect(ASD),andthetissuewasobtainedduringthesurgeryatthe WuhanChildrensHospitalrfomApril,1999toMarch,2000.Thettest andlinear-relationshipanalysiswereusedforstatisticalanalysis. Results:1)ChangesofSRCat+-ATPB,}mRNAexpressionlevel:The levelingroupAwassignificantlylowerthangroupBandC(P0.01). ThelevelingroupBwasmildlyhigherthangroupC,butwithout statisticalsignificance(P0.05). 2)ChangesofSRRyRmRNA expressi