Receptor-MediatedEnytosis受体介导的内吞作用.pptVIP

Receptor-Mediated Endocytosis受体介导的内吞作用 组员： 康艳春 张柳 Introduction Receptor mediated endocytosis(RME) is an endocytotic mechanism in which specific molecules are ingested into the cell. The specificity results from a receptor-ligand interaction. Receptors on the plasma membrane of the target tissue will specifically bind to ligands on the outside of the cell. An endocytotic process occurs and the ligand is ingested. In 1964,Thomas Roth and Keith Porter reported on the mechanism by which yolk proteins might be taken into the oocytes（卵母细胞）of mosquitoes 报道了蚊子的卵黄蛋白进入卵母细胞的可能机制 一项实验研究：提出问题→观察→推测 Raise the question: How are the high-molecular-weight yolk proteins able to enter the oocyte? 问题的提出： 高相对分子质量的卵黄蛋白如何进入卵黄细胞？ Notice: during stages of rapid oocyte growth, there was a dramatic increase in the number of pitlike depressions seen on the surface of the oocyte. 注意到：在卵黄细胞快速 生长的阶段，其细胞表面 小窝样凹陷的数目剧增， 这些小窝是由质膜的内陷 形成的，并且在小窝的内 表面覆盖着一层刚毛状的 外被。 Postulation: yolk proteins were specifically adsorbed onto the outer surface of the membrane of the coated pits , which would then invaginate as coated vesicles. 推测：卵黄蛋白特异地吸附在有被小窝膜的外表面，然后内陷为包被小泡。 这些小泡失去它们的刚毛状的外被，相互融合形成大的膜包被的卵黄体，这是成熟的卵黄细胞的特征。 The first insight into the structure of coated vesciles Electron microscopic examination: a crude vesicle fraction isolated from guinea pig brains（Figure1） 从豚鼠脑髓分离出粗制的小泡组分进行电镜观察 Show: coated vesicles were covered by a polygonal basketwork. 表明：包被小泡表面覆盖着多边形的编织物 Suggestion: the coatings were an apparatus to control the infolding of the plasma membrane during vesicle formation. 研究者提出：外被是在小泡形成过程中用来控制质膜折叠的装置 The first studies of the biochemical nature of the vesicle coat 方法：将来自猪脑的小泡经过连续的蔗糖密度梯度离心，直到获得纯化的包被小泡。包被小泡的蛋白质被溶解后经过SDS-聚丙烯酰胺凝胶电泳分离。 结果表明：外被含一种相对分子质量约为180000的优势蛋白质。 Pearse将其命名为网格蛋白 普遍性:他再从其他几种动物的不同类型的细胞中分离的包被小泡的样品中，基于相对分子质量和肽谱分析也发现了同样的蛋白质 Brown and Goldstein had become Interested in the inherited c