曲安奈德抑制BN大鼠脉络膜新生血管生长.docVIP

曲安奈德抑制BN大鼠脉络膜新生血管生长 【摘要】 目的：观察光凝后即刻玻璃体腔内注射曲安奈德(triamcinolone acetonide,TA)对BN大鼠脉络膜新生血管(choroidal neovascularization,CNV)生长的抑制作用机制。方法：健康BN大鼠36只随机分为两组，随机抽取一眼作为实验眼。建立BN大鼠CNV动物模型。光凝后的即刻行玻璃体腔内注射TA 8μL(320μg)，对照组则在光凝眼行玻璃体腔内注射BSS 8μL。分别在光凝后1，2，4wk各处死动物6只，摘除眼球，制作石蜡切片，利用光镜观察不同时间点的CNV的变化。利用免疫组化的方法对NFκB，MCP1和FⅧRAg蛋白的表达进行半定量检测，并用原位杂交法检测VEGF的基因表达，并行半定量检测。结果：实验1，2，4wk，与对照组相比NFκB，MCP1和FⅧRAg蛋白表达及VEGF的基因表达明显降低(Plt;0.05)。结论：TA能够降低NFκB，MCP1等的表达和VEGF的转录，故TA是治疗CNV的一种有效的药物。 【关键词】 曲安奈德;脉络膜新生血管;核因子κB;单核细胞趋化蛋白1;血管内皮生长因子;Ⅷ因子相关抗原;积分光密度 　　　AbstractAIM: To observe the inhibition of krypton induced choroidal neovascularization (CNV) of brown norway (BN) rat by intravitreal triamcinolone acetonide(TA) injection after photocoagulation, and to study the mechanism of inhibition of CNV growth.METHODS: Thirtysix healthy BN rats were divided randomly to two groups, one eye was chosen randomly as experimental eye. After establishing BN rat CNV model, 8μL(320μg) TA was injected in vitreous immediately after photocoagulation in treatment group, the same volume isotonic balanced salt solution (BSS) was injected in vitreous in the contrast group. Eyes of 6 BN rats were enucleated for histological slices in 1 week, 2,4 weeks. The protein expression of nuclear factorkappa B (NFκB), monocyte chemoattractant protein1(MCP1), factorⅧrelated antigen(FⅧRAg)was examined by immunohistochemical method, and the transcription of vascular endothelial growth factor mRNA(VEGFmRNA) was Examined by in situ hybridization, meanwhile semiquantitative analysis was conducted.RESULTS: In treatment group, the positive stain optical density of NFκB, MCP1, FⅧRAg, and VEGFmRNA was obviously lower the contemporaneous control group(Plt;0.05).CONCLUSION: TA can lower the expression of VEGF mRNA transcription and the protein expression of NFκB, MCP1 and FⅧRAg, so it can effectively inhibit the development of BN rat CNV, which indicats that TA is an effective drug to treat CNV. 　　KEYWORDS：triamcinolon