人类结肠癌裸鼠移植瘤模型制作及临床1.5TMR成像仪的1H-MRS研究.docVIP

人类结肠癌裸鼠移植瘤模型制作及临床1.5TMR成像仪的1H-MRS研究 【摘要】 目的 探索临床医用1.5T MR成像仪对人类结肠癌荷瘤裸鼠模型行常规MR成像及氢质子MR频谱（1H-MR spectroscopy，1H-MRS）分析的可能性。 方法 人类结肠癌SW480肿瘤块种植于25只麻醉状态下的裸鼠皮下制成结肠癌异位移植瘤裸鼠模型。给予荷瘤鼠行MR T1WI、T2WI、1H-MRS扫描，并与病理所见对比。 结果 25只裸鼠均成瘤。T2WI图像均能清晰显示肿瘤，肿瘤T1WI信号与肌肉等，T2WI为高信号，中央坏死区为更高信号。18只鼠MRS单体素采集成功，22只二维多体素采集成功，25只三维多体素采集成功。 结论 临床医用1.5TMR与临床医用线圈对人类结肠癌裸鼠模型可行1H-MRS研究，常规MRI T2WI图像可反映移植瘤的病理改变，研究结果更容易反映临床人类疾病的特点。 【关键词】 裸鼠 新生物 结肠 磁共振成像 氢质子MR频谱 [Abstract] Objective To investigate the feasibility of MR T1WI, T2WI and 1H-MR spectroscopy（1H-MRS）with clinical 1.5T scanner in vivo in a xenotransplant nude mouse model with human colon carcinoma. Methods The tumor pieces of human colon cancer SW480 were implanted subcutaneously in 25 nude mice. These mice were scanned with TSE-T1WI, T2WI and 1H-MRS and MR images were compared with histopathological findings. Results The subcutaneous tumors grew in 25 mice. All tumors were detected clearly on T2WI images, and shown as iso-intensity as muscle on T1WI and hyper-intensity on T2WI in which more hyper-intensity in necrotic areas. 1H-MRS were satisfactory for 18 mice with single voxel, 22 mice with 2-demensional multi voxel and 25 mice with 3-demensional multi voxel. Conclusion A xenotransplant nude mouse model with human colon carcinoma can be investigated with 1H-MRS at a clinic 1.5T scanner. MR images on T2WI correlated well with pathology findings, and may be advantages for easier extrapolation of the result to clinical human studies. [Key words] nude mouse; neoplasm; colon; magnetic resonance imaging; 1H-MR spectroscopy MR波谱分析（MR spectroscopy，MRS）是目前唯一活体无创显示肿瘤代谢的方法，用动物模型充分开发其潜能、探索MRS在肿瘤诊断、新的治疗药物的无创性疗效监测中的作用有重要实用价值。目前裸鼠模型的MRS采用高场或极高场（4.7T-8.5T）的小动物专用成像仪与动物专用线圈[1～3]。但小动物专用MR成像仪稀少, 难于满足日益增多的实验研究。因此，有必要探索采用临床医用MR成像设备行小鼠模型的1H-MRS的研究。 材料与方法 1. 动物模型制作 人类结肠癌SW480细胞株（p53突变型）购自中国科学院上海细胞库。常规细胞培养，取对数生长期细胞制成细胞浓度为1×107/ml细胞悬液。 BALB/c裸小鼠购自中山大学动物实验部，体重21±3g，雌雄不限，养殖于中山大学动物实验部SPF级实验室。取两只裸鼠，用安尔碘上背部皮肤消毒，将制成的