不同溶菌酶的提取、分离方法的比较及酶活力测定方法之探讨.docVIP

毕业论文 作 者： 学 号： 院 系： 专 业： 题 目： 指导者 评阅者： 2014 年 6 月 Abstract Abandoned eggshell is an untapped treasure for human，its potential value is difficult to estimate.So this experiment based on the previous research，In the use of raw materials and Waste recycling point of view to improve the method of the extraction of lysozyme. the polyacrylic acid precipitation method of extracting lysozyme from eggshell was studied. The extraction, removing impurity protein, dissociation, salting out a series of steps successfully from the eggshell extract the trace of lysozyme and protein identification, and influences of pH value, temperature, concentration of NaCl solution on the extraction in the process of test, determine the optimum extraction conditions :the best extraction pH 4.5,the best extraction temperature is 40℃, the mass fraction of NaCl optimal extraction agent for 2.0%,the optimum salting out pH 11,The experiment?adopts the?method of polyacrylamide gel electrophoresis separation of proteins to identificate of爈ysozyme,爐the?determination of enzyme activityof lysozyme燽y spectrophotometry,study牋on the爄nhibitory effect of爈ysozyme on燽oring燽acillus,and compared to the enzyme activity at different extraction conditions.The experimental results show that:爄n the 1000g爓aste爋f fresh爀ggs燾an extract?.801g,lysozyme,lysozyme燾an inhibit the growth of燘acillus subtilis,and lysozyme activity was highest in the optimal extraction conditions of extraction, is 15220U / mg. Key words ：Eggshell;?lysozyme;?polyacrylic acid;?enzyme activity 摘 要 I Abstract II 目 录 IV 第1章 绪 论 1 1.1 溶菌酶的性质及作用 1 1.2 国内外研究现状和发展趋势 2 1.3 溶菌酶分离纯化的方法 3 1.3.1 结晶法 4 1.3.2 色谱法 4 1.3.3 亲和分离法 5 1.3.4 反胶团萃取法 5 1.3.5 双水相萃取法 6 1.3.6 超滤法 6 1.4 课题研究目的和意义 6 1.5 本课题主要研究内容 7 第2章 溶菌酶的提取、鉴定和酶活力的测定 8 2.1 实验材料和药品 8 2.2 试验仪器 9 2.3 工艺流程 10 2.4 实验过程 10 2.4.1 溶菌酶的提取 10 2.