复方甘草甜素脂质体对人肝星状细胞凋亡和增殖的作用.pdfVIP

一 212 现代消化及介入诊疗 2013年 第 18卷 第4期 ModemDigestionIntervention2013，Vo1．18，No．4 · 论 著 · 复方甘草甜素脂质体对人肝星状细胞凋亡 和增殖的作用 叶红军 华军霞1 韦始亮z 卢腾飞 周 政 摘【要】目的 观察复方甘草甜素脂质体对体外培养人肝星状细胞(hepaticstellatecells，HSCs)凋亡和 增值的作用，探讨抗肝纤维化新药物剂型。方法 体外培养人HSCs，分组 ：①HSCs对照组；②脂质体对 照组：③复方甘草甜素对照组和对应浓度的复方甘草甜素脂质体组。体外培养24h，用流式细胞术检测 HSCs细胞的凋亡率，以及MTr法检测 HSCs细胞增殖抑制率。结果 ①复方甘草甜素脂质体诱导HSC 凋亡率高于相同浓度的复方甘草甜素对照组，前两组的HSC凋亡率明显高于脂质体对照组和HSCs对 照组。②复方甘草甜素及其脂质体作用后的HSCs增殖的抑制率明显高于两个对照组，复方甘草甜素脂 质体组高于复方甘草甜素对照组(P0．05)，抑制率的递增呈浓度依赖性 ；HSCs对照组和脂质体对照组 无明显差别(P0．05)。结论 复方甘草甜素脂质体促进HSCs凋亡及抑制HSCs增殖的作用较复方甘草 甜素明显增强，单纯脂质体对体外培养的HSCs的凋亡和增殖无明显影响。 【关键词】肝纤维化；复方甘草甜素脂质体；肝星状细胞；凋亡；增殖 Theeffectsofcompoundglycyrrhizin liposomeonapoptosisandproliferationofhumanhepaticstellate cellsYEHong-jun1．HUAJun—xial，WEIShi—liange,LUTeng-feii．ZHOUZheng1．1)ShenzhenHengsheng Hospital,SourthernMedicafUniversity，Shenzhen518102，China；2)Shenzhen PreventionandTreatment CenterforOccupationalDiseases，Shenzhen518102，China A【bstract】 ObjectiveToobservetheeffectsofcompoundglycyrrhizinliposomeonhteapoptosisand proliferationofhumanhepaticstellatecells(HSCs)，andtOexploreanewkindofpharmaceuticalofrm onhte treatmentofhepaticfibrosis．MethodsHSCswere incubated wiht compoundglycyrrhizinliposome．orcom— poundglycyrrhizin，orliposomealone，orblank for24hours．TheapoptoticrateofHSCswastested byFlow cytometry，andtheproliferativeinhibition rateofhteHSCswasdeterminedby MTT assay．Resets 1．The apoptotic rates of theHSCs inducedwith compound glycyrrhizin liposomeand compoundglycyrrhizin were significantlyhigherthan thatoftwocontrolgroups． Theapoptotic rate ofhteHSCsinduced with compoundglycyrrhizin liposomewashigherthanhtatwithhteSalTleconcenrtationhtecompoundglycyrrhizin groupfP0．05)．2．TheproliferativeinhibitionratesoftheHSCswereinducedwithcompoundglycyrrhizin andcompoundglycyrrhizinliposome werehigherthan hteothertwoconrtolgroups．Theprolife