P16 P53Cyclin D1在卵巢浆液性癌中的表达及意义.pdfVIP

388 中国肿瘤临床 2012年第39卷第7期 Pl6P53CycIinD1在卵巢浆液性癌中的表达及 意义 赵立武∞ 申 彦① 师宜荃① 刘 艳① 刘易欣① 摘要 目的：探讨卵巢高、低级别浆液性癌中P16蛋 白、P53蛋 白、CyclinD1蛋白的表达情况及意义。方法：应用免疫组化法 对45例卵巢浆液性癌 (低级别19例，高级别26例)及25例 卵巢交界性浆液性囊腺瘤组织进行P16、P53、CyclinD1蛋 白检测，并分 析其意义。结果：P53在低级别癌 中阳性表达率为5％，显著低于高级别癌中的69％(P0．05)，而CyclinD1在低级别癌中阳性表达 率为89％，显著高于高级别癌中的15％(P0．05)，P53和CyclinD1表现为负相关(r=0．211)。P16在低、高级别癌肿的阳性表达率分 别为84％和92％，高级别癌阳性率高于低级别癌，但差异无统计学意义 0．05)。结论 ：P53和CyclinD1在卵巢高、低级别浆液性 癌中的表达有显著差异，对于形态上介于高、低级别之间的浆液性癌，二者可能成为分级的辅助手段。 关键词 P16 P53 CyclinD1 卵巢肿瘤 浆液性癌 doi：10．3969／j．issn．1000—8179．2012．07．007 ExpressionofP16，P53，andCyclinD1ProteininLow-gradeandHigh-gradeOvarianSerous CarcinomaandItsClinicalSignificance LiwuZHAO ，YanSHEN ，YiquanSHI，YahLIU ，YixinLIU Correspondenceto：YixinLIU，E—mail：yixinliu66@yahoo．com．cn DepartmentofPathology,TianjinCentralHospitalofGynecologyandObstetricsofTianjinMedicalUniversity,Tianjin300100，China DepartmentofGynaecologyandObstetrics，NingheCountryHospital，Tianjin301500，China Abstract Objective：ToinvestigatetheexpressionofP16，P53，andCyclinD1proteininlow—gradeandhigh—gradeovarianse— rouscarcinomaanditsclinicalsignificance．Theclinicaldataandpathologicalfindingswerena alyzed．M ethods：Fourtyfivecasesof ovarianserouscarcinoma(19casesoflow．gradeand26casesofhigh．gradecarcinoma)nad25casesofborderlineovarianseroustu． morswereselected．ImmunohistochemicalstudyofP16，P53，andCyclinD1wasperformedinall70cases．Results：Thehigh—gradese- rouscarcinomahadasignificantlyhigherexpressionlevelofP53(69％versus5％)，whilelow—gradecarcinomahadasignificantly higherexpressionlevelofCyclinD1(89％versus15％)．SignificantdifferenceswereobservedintheexpressionbetweenP53andCy— clinD1．Aninversecorrelationexistedbewt eentheexpressionofP53andCyclinD1intumorgrade．r 0．211．Nodifferencewasob— servedintheexpressionofP16betweenlow—gradeandhigh—gradeovarianserouscarcinoma(84％versus92％)．Conclusion：P53