JNK在棕榈酸致骨骼肌细胞胰岛素抵抗中的作用.pdfVIP

天津医药2011 年 1 月第39 卷第 1 期 53 JNK 在棕榈酸致骨骼肌细胞胰岛素抵抗中的作用* 高 静 王 慧 牛文彦△ 摘要 目的:探讨棕榈酸造成L6GLUT4myc骨骼肌细胞胰岛素抵抗过程中c-Jun N-末端激酶(JNK)所起的作用。 方法:将L6GLUT4myc 成肌细胞培养于24 孔和6 孔培养板中，随机分为溶剂组和棕榈酸组，分别用0.3 mmol/L 的棕榈 酸盐和溶剂牛血清白蛋白（BSA）孵育16 h ，在棕榈酸组的后30 min 加入JNK 的抑制剂，于胰岛素刺激前后用酶联免疫 吸附测定（ELISA ）细胞膜上GLUT4myc的含量，免疫印迹法测定蛋白激酶B(Akt)、JNK 和胰岛素受体底物1（IRS1）的磷 结果： 酸化。 与溶剂组相比，棕榈酸组中胰岛素增加的GLUT4myc水平的倍数和Akt 的磷酸化降低(P 0.05) ；JNK 和 结论： IRS1 的丝氨酸位点S307 的磷酸化水平变化差异无统计学意义(P0.05) 。 棕榈酸导致L6 骨骼肌细胞胰岛素抵 抗的机制可能不涉及JNK ，或JNK 的作用很小。 关键词 棕榈酸 肌, 骨骼 葡萄糖转运体4 型 胰岛素 胰岛素抗药性 JNK 丝裂原活化蛋白激酶类 蛋白 激酶类 TheRoleofJNKinthePalmitate-InducedInsulinResistanceinSkeletalMuscleCells GAO Jing, WANG Hui, NIU Wenyan DepartmentofImmunology,TianjinMedicalUniversity,Tianjin300070,China Abstract Objective:To study the role of c-jun N-terminal kinase (JNK) in the mechanism of palmitate-induced insu⁃ lin resistance in L6GLUT4myc skeletal muscle cells. Methods:L6GLUT4myc myoblasts were incubated in 24 and 6 wells plates and divided into two groups, and treated with 0.3 mmol / L palmitate or solvent BSA for 16 h, respectively. JNK inhibi⁃ tor was added to the medium during the last 30 min incubation with palmitate or BSA. The amount of GLUT4mycon the cell surface was measured by enzyme linked immunosorbent assay (ELISA) and the phosphorylation of protein kinase B (Akt). JNK and insulin receptor substrate 1 (IRS1) were measured by immunoblotting in the absence or presence of insulin respec⁃ tively. Results:Compared to the solvent group, insulin-stimulated GLUT4myctranslocation and phosphorylation of Akt de⁃ creased in palmitate group (P 0.05). There was