临床研究优化的预包被式多探针荧光原位杂交技术可提高白血….pdfVIP

2012;32(10) 南方医科大学学报（J South Med Univ ） ·1457 · 临床研究 优化的预包被式多探针荧光原位杂交技术可提高白血病细胞遗 传学异常的检测效率 曹 睿，宋兰林，吴福群，廖立斌，左 媛，刘晓力 南方医科大学南方医院血液科实验室，广东 广州 510515 摘要：目的探讨对预包被式多探针荧光原位杂交（FISH）技术进行优化在检测白血病细胞遗传学异常的意义。方法 141例初 诊为ALL 或AML/MDS 的患者骨髓标本，行预优化的包被式多探针FISH 流程检测（在原始流程的基础上增加了调整细胞密度、 蛋白酶消化步骤），其中35 例行常规流程检测，对比优化前后对白血病细胞遗传学异常的检测成功率与阳性位点检出率。结果 对多探针FISH 实验流程进行优化后，ALL 的检测成功率由85.3%提高到100% ，阳性位点检出率由5.1%提高到8.6% ；AML/ MDS 的检测成功率由67.4%提高到99.8% ，阳性位点检出率由3.5%提高到6.0% ，差异均具有统计学意义（P0.01）。结论通过 对常规预包被式多探针FISH 系统检测流程进行优化，显著提高了白血病细胞遗传学异常的检测效率，增强了该技术的临床实 用性。 关键词：多探针FISH ；优化；细胞遗传学异常 中图分类号：R733.71 文献标志码：A 文章编号：1673-4254（2012）10-1457-04 doi: 10.3969/j.issn. 1673-4254.2012.10.016 /kcms/detail/44.1627.R2011.002.html Optimization of pre-coated multi-probe fluorescence in situ hybridization for cytogenetic detection of acute leukemia CAO Rui, SONG Lanlin, WU Fuqun, LIAO Libin, ZUO Yuan, LIU Xiaoli Hematology Laboratory, Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To optimize pre-coated multiple-probe fluorescence in situ hybridization (FISH) to improve its efficiency in cytogenetic diagnosis of acute leukemia. Methods The original multiple-probe FISH techniques were optimized by adjusting the cell density and adding a process of protease digestion. Cytogenetic anomalies were detected in 141 patients with acute lymphocytic leukemia (ALL) or acute myeloid leukemia/ myelodysplastic syndromes (AML/MDS) using the modified technique, and 35 of the patients were also examined using the original technique. The successful detection rate and positive site detection rate were compared between the modified and original techniques. Results Modification of the pre-coated multiple-probe FISH technique resulted in an significant i