C反应蛋白诱导内皮细胞炎症相关因_省略_iNOS及ICAM_1表达的研究_宋旭东.pdfVIP

2011 8 1 3 8 Med J Chin PLA, Vol1 3 , No1 8, August 1, 2011 # 821 # # # C反应蛋白诱导内皮细胞炎症相关因子iNOS 及ICAM- 1 表达的研究 宋旭东, 陈爱华, 何非, 李志梁,刘映峰 [ ] C ( CRP) ( iNOS) - 1( ICAM- 1) 151020mg/ L CRP , ( HUVEC) CRP RT- PCR CRP HUVEC ICAM- 1 mRNA ; ELISA 1520mg/ L CRP HUVEC ICAM- 1iNOS RT-PCR ICAM- 1 mRNA , 1510mg/ L CRP , ICAM- 1 mRNA , 20mg/ L CRP ICAM- 1 mRNA ( 11 125) (01 7 0) 1148 ELISA 15mg/ L CRP ICAM-1 (P 01 05) , 20mg/ L CRP , ICAM- 1 (P 01 01) ; 15mg/ L CRP , iNOS (P 01 05) , 20mg/ LCRP , iNOS (P 01 01) CRP , ( 20mg/ L) [ ] C ; ; 1; [ ] R54114 [ ] A [ ] 0577-7402(2011) 08-0821-04 Expression of inflammation related factors iNOS and ICAM- 1 in endothelial cells induced by C-reactive protein * SONG Xu-dong, CHEN A-i hua , HE Fei, LI Zh-i liang, LIU Ying-feng1 Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China * Corresponding author, E-mail: chenaha@gmail1 com [Abstract Objective To investigate the expression of inducible nitric oxide synthase ( iNOS) and intercellular cell adhesion molecule- 1 ( ICAM- 1) in endothelial cells induced by C-reactive protein ( CRP) and its corresponding mechanisms. Methods Human umbilical cord vein endothelial cells ( HUVEC) were treated with different concentrations of CRP or with phosphate buffered solution as control, and RT-PCR was used for measurement of the expression of ICAM- 1 mRNA induced by CRP in HUVECs. H UVECwere treated with CRP of 1mg/ L, 5mg/ L, 20mg/ L, or with phosphate buffered solution, and expressions of ICAM- 1 and iNOS protein in HUVECs were detected by cellular enzyme linked immunosorbent assay ( ELISA) . Results In groups of 1mg/ L, 5mg/ L and 10mg/ L CRP, no different effec