壬基酚对秀丽隐杆线虫的生殖毒性研究.pdfVIP

analogues: Structure-activity relationship and influence of microenvironment[J].Food Chemistry, 2010,119:1435-1442. [24] Stig B, 刘青.植物源保护剂姜黄素的研究进展[J].现代药物与临床, 2009, 24(1) : 22-31. 作者简介：黄真真，四川大学华西公共卫生学院2013级硕士研究生，研究方向：食品毒理学及保健品功 能评价 壬基酚对秀丽隐杆线虫的生殖毒性研究 杨栋 刘冉 尹立红 浦跃朴5 东南大学公共卫生学院环境医学工程教育部重点实验室，南京 210009 摘要： 目的 应用模式生物－秀丽隐杆线虫研究壬基酚(NP)的生殖毒性。方法 采用七个不同浓度的 壬基酚(100、10、1、0.1、0.01、0.001 mg/L、溶剂对照、空白对照)染毒 24h ，应用线虫的LC50 、后 代数目、世代时间和精子发生相关基因(spe-10、spe-15、folt-1、fer-1)等指标对 NP 毒性作用进行综 合评价。结果 急性毒性试验中线虫存活数目在 NP 暴露的各个剂量组与对照组之间均没有统计学差 异(p0.05)，线虫的后代数目在各个剂量组之间没有统计学差异(p0.05)，均数在 132-163 个之间， NP 暴露组与空白对照组的世代时间相比较有统计学差异(p0.05)，最高剂量组与空白组比较世代时 间差小于 3h，精子发生四个基因的表达水平均有所下降，其中 spe-10 基因，染毒组与对照组比较有 统计学差异(p0.05) 。结论 NP 影响线虫精子发生相关基因的表达,对后代数目和世代时间影响较小， 运用线虫模型评价 NP 的生殖毒性，其基因表达的改变先于表型指标。 关键词：壬基酚；生殖毒性；秀丽隐杆线虫 The reproductive toxicity of Nonylphenol on Caenorhabditis elegans Yang Dong, Liu Ran, Yin Lihong ,Pu Yuepu School of Public Health, Southeast University (Nanjing 210009, China ) Abstract: objective To evaluate the reproductive toxicity of Nonylphenol (NP) on model organisms-Caenorhabditis elegans. Method C. elegans were exposed to seven different concentrations of nonylphenol (100, 10, 1, 0.1, 0.01, 0.001 mg/l, solvent control, control) for 24 hour, The toxic effects were determined with endpoints of LC50, brood size, generation time and spermatogenesis-related genes (spe-10, spe-15, folt-1, fer-1). Results The survived numbers have no statistics difference between all dose group and controlled group in acute toxicity test (p0.05). the exposure of all dose of NP, brood size was not decrease with statistics differences (p0.05), the brood size were between 132-163. The generation times were significantly lengthened when C. elegans were exposed to NP (p0.05), but the generation time is less t