病原菌TAL效应子与寄主靶基因相互识别分子密码.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2011, August 25; 27(8): 1132−1141 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2011 CJB, All rights reserved. 综 述 病原菌TAL 效应子与寄主靶基因相互识别的分子密码 李岩强，王春连，赵开军 中国农业科学院作物科学研究所 农业部作物遗传育种重点实验室 农作物基因资源与基因改良国家重大科学工程，北京 100081 摘 要: 黄单胞杆菌属 TAL 效应子类蛋白作为病原菌的毒性因子或无毒因子，能够与寄主靶基因 DNA 的启动子进行 特异性识别，调控寄主的基因表达，引起致病或抗病反应。TAL 效应子类蛋白识别靶基因DNA 的模式，是2 个氨基酸 决定 1 个核苷酸的识别。这种新型的蛋白质-DNA 互作方式有可能在基因治疗、植物抗病基因发掘、广谱抗病基因构建 等生物医学工程和农业工程方面得到广泛应用。文中综述了TAL 效应子类蛋白的发现及功能，TAL 效应子与寄主靶基 因识别的专一性及分子密码，并对该分子密码当前的应用现状及前景进行了讨论和展望。 关键词: TAL 效应子，分子识别密码，蛋白质-DNA 互作，基因治疗，抗病育种 Molecular recognition code between pathogenic bacterial TAL-effectors and host target genes: a review Yanqiang Li, Chunlian Wang, and Kaijun Zhao Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences , Chinese Academy of Agricultural Sciences, Beijing 100081, China Abstract: As the pathogenic bacterial virulence and avirulence factors, transcription activator like (TAL) effectors of Xanthomonas can resulted in the host diseases or resistance responses. TAL effectors can specifically bind the target DNA of host plant with a novel protein-DNA binding pattern in which two amino acids recognize one nucleotide. The complexities of TAL-DNA binding have the feasibility in use of gene therapy through homologous recombination and site-specific mutation. By using the molecular recognition code between TAL-effectors and host target genes, we can exploit both the susceptible and resistance genes; broad spectrum resistance induced by multiple TAL effectors could also be manipulated. Deeper insight in t