玉米大斑病抗性基因Ht1定位区域内候选序列的生物信息学分析.pdfVIP

HEREDITAS (Beijing) 2009 6 , 31(6): 638―644 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2009.00638 玉米大斑病抗性基因Ht1 定位区域内候选序列的生物 信息学分析 鄂洋, 林凤, 张春宇, 崔娜, 许玉凤 , 110161 为获得玉米大斑病抗性基因Ht1 候选序列, 文章采用生物信息学方法对与玉米大斑病抗性基因Ht1 紧密 连锁的分子标记umc22 和umc 122 定位区域内候选序列进行了分析, 其中得到的63 条ORF 序列中有 14 条序列 可编码蛋白质结构域。将 14 条核苷酸酸序列预测出的氨基酸序列与已克隆的24 条抗性基因编码氨基酸序列进 行 Blast 比对及进化树构建。结果发现, 候选序列 gpm565a 具有植物抗性基因编码产物的高度保守结构域, 而 且与抗性基因Xal 相似性高、亲缘关系近, 推测可能与抗性基因Ht1 有关。其他候选序列由于不具有植物抗性 基因编码产物的高度保守结构域或者相似性低、亲缘关系远等原因, 不能确定与抗性基因Ht1 有关。通过对候 选序列 gpm565a 进行二级结构及三维结构分析, 发现有大量构成蛋白质特异功能结构组件的无规则卷曲存在, 推测gpm565a 可能是Ht1 功能域的一部分。 玉米大斑病; 候选序列; 抗性基因 Bioinformatics analysis of candidate sequences between two flanking markers linked with resistance gene Ht1 to Exserohilum turcicum in maize E Yang, LIN Feng, ZHANG Chun-Yu, CUI Na, XU Yu-Feng Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110161, China Abstract: In order to get Ht1 candidate sequence(s), bioinformatics method was employed to analyze sequences between two flanking markers umc22a and umc 122a linked with Ht1 . Sixty-three open reading frame (ORF) sequences were found, fourteen of which encoded protein domains. The amino acid sequences encoded by these ORF sequences were blasted with the 24 cloned resistance gene amino acid sequences, and a phylogenetic tree was constructed. Among the fourteen ORF sequences, gpm565a with nucleotide binding site (NBS), containing a lot of random coils, had both high identity and close relationship with Xal resistant to bacterial blight, and was predicted to be related with Ht1 . The remaining thirteen ORF sequences had no enough evidence to show any relationships with Ht1 due to lack of conserved domains, low identity or distant