糖皮质激素及灌注色氨酸对肉仔鸡下丘脑代谢物变化的影响.pdfVIP

中国农业科学 2010,43(16):3440-3446 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.16.021 糖皮质激素及灌注色氨酸对肉仔鸡下丘脑代谢物变化的影响 孔 雪，宋志刚，焦洪超，林 海 （山东农业大学动物科技学院，山东泰安 271018 ） 摘要：【目的】利用皮下注射人工合成糖皮质激素的方式模拟应激，通过核磁共振（NMR）技术研究应激及色 氨酸对肉仔鸡下丘脑代谢状况的影响，为探明下丘脑食欲调控机制提供依据。【方法】10 日龄 AA 肉仔鸡 32 只随 机分为 4 组，早 6：00 禁食 3h 后，以 2.0 mg·kg-1BW 剂量皮下注射地塞米松，同时灌注色氨酸（生理盐水溶解， 500 mg·kg-1BW），对照组分别皮下注射、灌服生理盐水，3h后取血浆及下丘脑。下丘脑用高氯酸萃取后进行核磁共 1 振研究。【结果】通过H NMR谱，发现应激改变了肉鸡下丘脑乳酸，葡萄糖，ATP，肌醇，3-羟基丁酸的相对含量； 未检测到色氨酸信号的改变。【结论】NMR 技术能够检测到应激状态下下丘脑代谢物的改变；应激状态下，ATP、 葡萄糖和其它能量底物信号的改变可能参与了下丘脑对食欲的调控作用。 关键词：应激；肉仔鸡；食欲；色氨酸；下丘脑；核磁共振 Effects of Glucocorticoid and Tryptophan Perfusion on Hypothalamic Metabolites of Broilers KONG Xue, SONG Zhi-gang, JIAO Hong-chao, LIN Hai (College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, Shandong) Abstract: 【Objective 】 The effects of glucocorticoid and tryptophan on the hypothalamic metabolites concentration of broiler chickens were investigated by nuclear magnetic resonance (NMR) in the present study. The aim of the present study was to provide evidences for the understanding of hypothalamic regulation mechanism on appetite and expenditure at stress status. 【Method 】 Thirty-two 10-day-old chickens were divided into 4 groups randomly, dexamethasone (DEX, 2.6 mg·kg-1 BW) was injected subcutaneously and tryptophan (500 mg·kg-1 BW) was oral administrated after 3 h fasting, while the control chickens were subjected to saline administration, respectively. Blood and hypothalamus were obtained and hypothalamus extraction was used for NMR analysis. 【Result 】 The 1H NMR spectra showed